Anti-ATF2 antibody (ab65490)

Overview

• Product nameAnti-ATF2 antibodySee all ATF2 primary antibodies ...
• Description
  Rabbit polyclonal to ATF2
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S94 of human ATF2.

• Positive control293 cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was purified through a protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
• Tissue specificityAbundant expression seen in the brain.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
  Contains 1 C2H2-type zinc finger.
• Post-translational
  modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
• Cellular localizationNucleus.

Target information above from: UniProt accession P15336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1386 Human
  • Omim: 123811 Human
  • SwissProt: P15336 Human
  • Unigene: 592510 Human

• Alternative names
  Activating Transcription Factor 2 antibodyActivating transcription factor 2 splice variant ATF2 var2 antibodyATF 2 antibody

  Atf-2 antibodyAtf2 antibodyATF2 protein antibodyATF2_HUMAN antibodycAMP Response Element Binding Protein 2 antibodycAMP response element binding protein CRE BP1 antibodycAMP response element-binding protein CRE-BP1 antibodycAMP responsive element binding protein 2, formerly antibodycAMP-dependent transcription factor ATF-2 antibodycAMP-responsive element-binding protein 2 antibodyCRE BP1 antibodyCRE-BP antibodyCREB 2 antibodyCREB-2 antibodyCREB2 antibodyCREBP1 antibodyCyclic AMP dependent transcription factor ATF 2 antibodyCyclic AMP-dependent transcription factor ATF-2 antibodyCyclic AMP-responsive element-binding protein 2 antibodyD130078H02Rik antibodyD18875 antibodyHB 16 antibodyHB16 antibodyMGC105211 antibodyMGC105222 antibodyMGC111558 antibodyMGC142504 antibodymXBP antibodyMXBP protein antibodyTg(Gzma-Klra1)7Wum antibodyTREB 7 antibodyTREB7 antibody

  see all

Anti-ATF2 antibody images

• 

  Western blot - ATF2 antibody (ab65490)
  All lanes : Anti-ATF2 antibody (ab65490) at 1/50 dilution
  
  Lane 1 : 293 cell lysate, nontransfected
  Lane 2 : 293 cell lysate, transiently transfected with the ATF2 gene
  
  Lysates/proteins at 2 µg per lane.
  
  
  Predicted band size : 52 kDa
  Observed band size : 57 kDa (why is the actual band size different from the predicted?)