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Western blot - ATF2 antibody (ab809)

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Product Name
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ATF2 antibody
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See all ATF2 antibodies (30)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ATF2
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Immunogen
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Synthetic peptide: IVMAPSSQSQPSGS, corresponding to C terminal amino acids 492-505 of Human ATF2. BLAST 'C-IVMAPSSQSQPSGS' with or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Rat (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 0.5 - 2 µg/ml. Detects bands of approximately 60 kDa and 28 kDa in HeLa cell lysates (predicted molecular weight: 54 kDa). Overnight incubation is recommended with this antibody.
Please note that the appox 28KDa band we observe maybe explained by the isoform described in Bailey et al [J. Clin. Endocr. Metab. 87: 1717-1728, 2002].(predicted molecular weight: 54 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysates
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Cellular localization
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Nuclear
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Relevance
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ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATF2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATF2 antibody images, references, products related to ab809 and other tools.
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ATF2 antibody images:
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Western blot - ATF2 antibody (ab809)
Predicted band size : 54 kDa
staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
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Search PubMed (MEDLINE) for references to ATF2
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ATF2 antibody - more information
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Customer reviews (feedback) regarding ATF2 antibody |
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