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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-ATF2 antibody
See all ATF2 products (32) ...
Description
Goat polyclonal to ATF2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Rat
Immunogen
Synthetic peptide: IVMAPSSQSQPSGS, corresponding to C terminal amino acids 492-505 of Human ATF2.
C-IVMAPSSQ SQPSGS
Positive control
HeLa cell lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Western blot - ATF2 antibody (ab809)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).(Overnight incubation is recommended.)
Target
Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificity
Abundant expression seen in the brain.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
Post-translational
modifications
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15336
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CREB 2 antibody
- HB 16 antibody
- Activating Transcription Factor 2 antibody
see all
Database links
- Entrez Gene: 1386 Human
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: Q00969 Rat
- Unigene: 592510 Human
- Unigene: 9825 Rat
Anti-ATF2 antibody images:
Western blot - ATF2 antibody (ab809)
Predicted band size : 54 kDa
staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
References for Anti-ATF2 antibody (ab809)
ab809 has not yet been referenced specifically in any publications.
Publishing research using ab809? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"