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Anti-ATF2 antibody (ab809)

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Overview

Product name

Anti-ATF2 antibody
See all ATF2 products (32) ...

Description

Goat polyclonal to ATF2

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Rat

Immunogen

Synthetic peptide: IVMAPSSQSQPSGS, corresponding to C terminal amino acids 492-505 of Human ATF2.

C-IVMAPSSQ SQPSGS

Positive control

HeLa cell lysates

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - ATF2 antibody (ab809)Western blot - ATF2 antibody (ab809) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).(Overnight incubation is recommended.)

Target

Function

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.

Tissue specificity

Abundant expression seen in the brain.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.

Post-translational
modifications

Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP15336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CREB 2 antibody
  • HB 16 antibody
  • Activating Transcription Factor 2 antibody
  • ATF 2 antibody
  • Atf-2 antibody
  • Atf2 antibody
  • ATF2 protein antibody
  • ATF2_HUMAN antibody
  • cAMP Response Element Binding Protein 2 antibody
  • cAMP response element binding protein CRE BP1 antibody
  • cAMP response element-binding protein CRE-BP1 antibody
  • cAMP-dependent transcription factor ATF-2 antibody
  • cAMP-responsive element-binding protein 2 antibody
  • CRE BP1 antibody
  • CRE-BP antibody
  • CREB 2 antibody
  • CREB-2 antibody
  • CREB2 antibody
  • CREBP1 antibody
  • Cyclic AMP dependent transcription factor ATF 2 antibody
  • Cyclic AMP-dependent transcription factor ATF-2 antibody
  • Cyclic AMP-responsive element-binding protein 2 antibody
  • D130078H02Rik antibody
  • D18875 antibody
  • HB 16 antibody
  • HB16 antibody
  • MGC105211 antibody
  • MGC105222 antibody
  • MGC111558 antibody
  • MGC142504 antibody
  • mXBP antibody
  • MXBP protein antibody
  • Tg(Gzma-Klra1)7Wum antibody
  • TREB 7 antibody
  • TREB7 antibody
see all

Anti-ATF2 antibody images:

  Western blot - ATF2 antibody (ab809)

Western blot - ATF2 antibody (ab809)



Predicted band size : 54 kDa


staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

References for Anti-ATF2 antibody (ab809)

ab809 has not yet been referenced specifically in any publications.

Publishing research using ab809? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"