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Product name
Anti-ATF2 (phospho S490 + S498) antibody
See all ATF2 products (33) ...
Description
Rabbit polyclonal to ATF2 (phospho S490 + S498)
Specificity
This antibody is specific for ~74 kDa ATF2 protein phosphorylated at Ser 490/498. The antibody also recognizes the phosphorylated ~54 kDa splice form of ATF2.
Tested applications
IHC-Fr, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser490/498 of ATF2 (Human).
Positive control
Human melanoma cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None.
Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/1000.IHC-Fr: 1/1000.
WB: 1/1000. Predicted molecular weight: 52 kDa.
Target
Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificity
Abundant expression seen in the brain.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
Post-translational
modifications
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15336
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CREB 2 antibody
- HB 16 antibody
- Activating Transcription Factor 2 antibody
see all
Database links
- Entrez Gene: 1386 Human
- Omim: 123811 Human
- SwissProt: P15336 Human
- Unigene: 592510 Human
References for Anti-ATF2 (phospho S490 + S498) antibody (ab22186)
ab22186 has not yet been referenced specifically in any publications.
Publishing research using ab22186? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"