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Anti-ATF2 (phospho S62 + S44) antibody (ab28847)

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Overview

Product name

Anti-ATF2 (phospho S62 + S44) antibody
See all ATF2 products (33) ...

Description

Rabbit polyclonal to ATF2 (phospho S62 + S44)

Specificity

ab28847 recognises Phospho-ATF-2(Ser44)

Tested applications

ELISA, IP, IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phosphopeptide derived from human ATF-2 around the phosphorylation site of Serine 62 or 44.

Positive control

HeLa lysate; Breast carcinoma.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

  • Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody (ab28847)Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody (ab28847) image (enlarge)

  • Western blot - ATF2 (phospho S62 / 44) antibody (ab28847)Western blot - ATF2 (phospho S62 / 44) antibody (ab28847) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/20000.

    ELISA: 1/20000.

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000. Dete...Read more →

    WB: 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

Function

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.

Tissue specificity

Abundant expression seen in the brain.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.

Post-translational
modifications

Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP15336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CREB 2 antibody
  • HB 16 antibody
  • Activating Transcription Factor 2 antibody
  • Activating Transcription Factor 2 antibody
  • ATF 2 antibody
  • Atf-2 antibody
  • Atf2 antibody
  • ATF2 protein antibody
  • ATF2_HUMAN antibody
  • cAMP Response Element Binding Protein 2 antibody
  • cAMP Response Element Binding Protein 2 antibody
  • cAMP response element binding protein CRE BP1 antibody
  • cAMP response element-binding protein CRE-BP1 antibody
  • cAMP-dependent transcription factor ATF-2 antibody
  • cAMP-responsive element-binding protein 2 antibody
  • CRE BP1 antibody
  • CRE-BP antibody
  • CREB 2 antibody
  • CREB-2 antibody
  • CREB2 antibody
  • CREB2 antibody
  • CREBP1 antibody
  • CREBP1 antibody
  • Cyclic AMP dependent transcription factor ATF 2 antibody
  • Cyclic AMP-dependent transcription factor ATF-2 antibody
  • Cyclic AMP-responsive element-binding protein 2 antibody
  • D130078H02Rik antibody
  • D18875 antibody
  • HB 16 antibody
  • HB16 antibody
  • HB16 antibody
  • MGC105211 antibody
  • MGC105222 antibody
  • MGC111558 antibody
  • MGC142504 antibody
  • mXBP antibody
  • mXBP antibody
  • MXBP protein antibody
  • Tg(Gzma-Klra1)7Wum antibody
  • TREB 7 antibody
  • TREB7 antibody
  • TREB7 antibody
see all

Database links

see all

Anti-ATF2 (phospho S62 + S44) antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody (ab28847)

Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody (ab28847)

ab28847 immunohistochemistry staining paraffin embedded breast carcinoma.

  Western blot - ATF2 (phospho S62 / 44) antibody (ab28847)

Western blot - ATF2 (phospho S62 / 44) antibody (ab28847)

All lanes : Anti-ATF2 (phospho S62 + S44) antibody (ab28847)

Lane 1 : Hela Lysate with Immunizing peptide
Lane 2 : Hela Lysate


Predicted band size : 52 kDa

References for Anti-ATF2 (phospho S62 + S44) antibody (ab28847)

ab28847 has not yet been referenced specifically in any publications.

Publishing research using ab28847? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"