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Product name
Anti-ATF2 (phospho S94) antibody
See all ATF2 products (33) ...
Description
Rabbit polyclonal to ATF2 (phospho S94)
Specificity
ab28814 recognises Phospho-ATF-2(Ser94).
Tested applications
IHC-P, IP, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phospho-peptide derived from human ATF-2 around the phosphorylation site of Serine 94.
Positive control
Breast carcinoma
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Immunohistochemistry (Paraffin-embedded sections) - ATF2 (phospho S94) antibody (ab28814)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
Target
Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificity
Abundant expression seen in the brain.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
Post-translational
modifications
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15336
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CREB 2 antibody
- HB 16 antibody
- Activating Transcription Factor 2 antibody
see all
Database links
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- Unigene: 592510 Human
- Unigene: 209903 Mouse
Anti-ATF2 (phospho S94) antibody images:
References for Anti-ATF2 (phospho S94) antibody (ab28814)
ab28814 has not yet been referenced specifically in any publications.
Publishing research using ab28814? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"