Anti-ATF2 (phospho T69 + T51) antibody (ab131106)

Overview

• Product nameAnti-ATF2 (phospho T69 + T51) antibodySee all ATF2 primary antibodies ...
• Description
  Rabbit polyclonal to ATF2 (phospho T69 + T51)
• Specificityab131106 detects endogenous levels of ATF2 only when phosphorylated at threonine 69 or 51.
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Threonine 69 or 51 (D-Q-T(p)-P-T) of Human ATF2 (UniProt P15336).

  Run BLAST with Run BLAST with
• Positive control3T3 cell extract treated with Anisomycin; HeLa cells; Human breast carcinoma tissue

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: PBS is without Mg²⁺ and Ca²⁺
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab131106 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
• Tissue specificityAbundant expression seen in the brain.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
  Contains 1 C2H2-type zinc finger.
• Post-translational
  modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
• Cellular localizationNucleus.

Target information above from: UniProt accession P15336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1386 Human
  • Entrez Gene: 100047997 Mouse
  • Entrez Gene: 11909 Mouse
  • Entrez Gene: 81647 Rat
  • Omim: 123811 Human
  • SwissProt: P15336 Human
  • SwissProt: P16951 Mouse
  • SwissProt: Q00969 Rat

  • Unigene: 592510 Human
  • Unigene: 209903 Mouse
  • Unigene: 9825 Rat

  see all

• Alternative names
  Activating transcription factor 2 antibodyActivating transcription factor 2 splice variant ATF2 var2 antibodyATF 2 antibody

  Atf-2 antibodyAtf2 antibodyATF2 protein antibodyATF2_HUMAN antibodycAMP Response Element Binding Protein 2 antibodycAMP response element binding protein CRE BP1 antibodycAMP response element-binding protein CRE-BP1 antibodycAMP responsive element binding protein 2, formerly antibodycAMP-dependent transcription factor ATF-2 antibodycAMP-responsive element-binding protein 2 antibodyCRE BP1 antibodyCRE-BP antibodyCREB 2 antibodyCREB-2 antibodyCREB2 antibodyCREBP1 antibodyCyclic AMP dependent transcription factor ATF 2 antibodyCyclic AMP-dependent transcription factor ATF-2 antibodyCyclic AMP-responsive element-binding protein 2 antibodyD130078H02Rik antibodyD18875 antibodyHB 16 antibodyHB16 antibodyMGC105211 antibodyMGC105222 antibodyMGC111558 antibodyMGC142504 antibodymXBP antibodyMXBP protein antibodyTg(Gzma-Klra1)7Wum antibodyTREB 7 antibodyTREB7 antibody

  see all

Anti-ATF2 (phospho T69 + T51) antibody images

• 

  Western blot - Anti-ATF2 (phospho T69 + T51) antibody (ab131106)
  All lanes : Anti-ATF2 (phospho T69 + T51) antibody (ab131106) at 1/500 dilution
  
  Lane 1 : 3T3 cell extract untreated
  Lane 2 : 3T3 cell extract treated with Anisomycin
  
  
  Predicted band size : 55 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 (phospho T69 + T51) antibody (ab131106)
  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ATF2 (phospho T69 or T51) with ab131106 at 1/50 dilution. Right panel was preincubated with blocking peptide.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T69 + T51) antibody (ab131106)
  Immunofluorescence analysis of methanol-fixed HeLa cells staining ATF2 (phospho T69 or T51) with ab131106 at 1/100 dilution.