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Product name Anti-ATF2 (phospho T69 + T51) antibody See all ATF2 primary antibodies
Rabbit polyclonal to ATF2 (phospho T69 + T51)
Specificity ab131106 detects endogenous levels of ATF2 only when phosphorylated at threonine 69 or 51.
Tested applications WB, IHC-P, ICC/IF more details
Mouse, Rat, Human
Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Threonine 69 or 51 (
D-Q-T(p)-P-T ) of Human ATF2 (UniProt P15336).
3T3 cell extract treated with Anisomycin; HeLa cells; Human breast carcinoma tissue
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride Note: PBS is without Mg 2+ and Ca 2+
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab131106 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Predicted molecular weight: 55 kDa.
1/50 - 1/100.
1/100 - 1/200.
Function Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificity Abundant expression seen in the brain.
Sequence similarities Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger.
Post-translational modifications Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cellular localization Nucleus.
Information by UniProt
Activating transcription factor 2 antibody
Activating transcription factor 2 splice variant ATF2 var2 antibody
ATF 2 antibody
Anti-ATF2 (phospho T69 + T51) antibody images
Western blot - Anti-ATF2 (phospho T69 + T51) antibody (ab131106)
All lanes : Anti-ATF2 (phospho T69 + T51) antibody (ab131106) at 1/500 dilution Lane 1 : 3T3 cell extract untreated Lane 2 : 3T3 cell extract treated with Anisomycin Predicted band size : 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 (phospho T69 + T51) antibody (ab131106)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ATF2 (phospho T69 or T51) with ab131106 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T69 + T51) antibody (ab131106)
Immunofluorescence analysis of methanol-fixed HeLa cells staining ATF2 (phospho T69 or T51) with ab131106 at 1/100 dilution.
References for Anti-ATF2 (phospho T69 + T51) antibody (ab131106)
This product has been referenced in:
et al. Inhibitory effect of baicalin on iNOS and NO expression in intestinal mucosa of rats with acute endotoxemia. PLoS One 8:e80997 (2013).
Read more (PubMed: 24312512) »
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for this product
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