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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Anti-ATF2 (phospho T69 + T51) antibody (ab28848)
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Immunohistochemistry (Paraffin-embedded sections) - ATF2 (phospho T69/51) antibody (ab28848)
Ab28848, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma by Immunohistochemistry.
Left image: using ab28848, phosphorylated ATF2 (Thr69/51) antibody.
Right image: the same antibody preincubated with synthesized phosphopeptide.
Western blot - ATF2 (phospho T69/51) antibody (ab28848)
All lanes : Anti-ATF2 (phospho T69 + T51) antibody (ab28848)
Lane 1 : LOVO cell lysate (5-30ug).
Lane 2 : LOVO cell lysate (5-30ug). with Immunizing peptide
Predicted band size : 52 kDa
Observed band size : 52 kDa
Western blot - ATF2 (phospho T69/51) antibody (ab28848)
Anti-ATF2 (phospho T69 + T51) antibody (ab28848) at 1/750 dilution
Secondary
HRP conjugated sheep anti-rabbit IgG polyclonal at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
This image is courtesy of an anonymous Abreview
ATF2 (phospho T69/51) antibody for WB in Human (28848)
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