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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-ATF2 (phospho T69 + T51) antibody
See all ATF2 products (32) ...
Description
Rabbit polyclonal to ATF2 (phospho T69 + T51)
Tested applications
ELISA, IHC-P, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 69/51.
Positive control
WB: LOVO cell lysate. IHC-P: breast carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000.Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Target
Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificity
Abundant expression seen in the brain.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
Post-translational
modifications
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15336
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CREB 2 antibody
- HB 16 antibody
- Activating Transcription Factor 2 antibody
see all
Database links
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- SwissProt: Q00969 Rat
see all
Anti-ATF2 (phospho T69 + T51) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - ATF2 (phospho T69/51) antibody (ab28848)
Ab28848, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma by Immunohistochemistry.
Left image: using ab28848, phosphorylated ATF2 (Thr69/51) antibody.
Right image: the same antibody preincubated with synthesized phosphopeptide.
Western blot - ATF2 (phospho T69/51) antibody (ab28848)
All lanes : Anti-ATF2 (phospho T69 + T51) antibody (ab28848)
Lane 1 : LOVO cell lysate (5-30ug).
Lane 2 : LOVO cell lysate (5-30ug). with Immunizing peptide
Predicted band size : 52 kDa
Observed band size : 52 kDa
Western blot - ATF2 (phospho T69/51) antibody (ab28848)
Anti-ATF2 (phospho T69 + T51) antibody (ab28848) at 1/750 dilution
Secondary
HRP conjugated sheep anti-rabbit IgG polyclonal at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
This image is courtesy of an anonymous Abreview
References for Anti-ATF2 (phospho T69 + T51) antibody (ab28848)
This product has been referenced in:
- Diring Jet al. A Cytoplasmic Negative Regulator Isoform of ATF7 Impairs ATF7 and ATF2 Phosphorylation and Transcriptional Activity. PLoS One 6:e23351 (2011). WB; Human.Read more (PubMed: 21858082) »
See 1 publication for this product
Publishing research using ab28848? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"