Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Primary antibodies
- Secondary antibodies
- Slides
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-ATF2 (phospho T71 + T53) antibody
See all ATF2 products (33) ...
Description
Rabbit polyclonal to ATF2 (phospho T71 + T53)
Tested applications
WB, IHC-P, ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthesized phospho peptide derived from human ATF2 around the phosphorylation site of Threonine 71 or 53.
Positive control
IHC-P: Human breast carcinoma. WB: HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IP: Use at an assay dependent dilution.
Target
Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificity
Abundant expression seen in the brain.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
Post-translational
modifications
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15336
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CREB 2 antibody
- HB 16 antibody
- Activating Transcription Factor 2 antibody
see all
Database links
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- Unigene: 592510 Human
- Unigene: 209903 Mouse
Anti-ATF2 (phospho T71 + T53) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - ATF2 (phospho T53) antibody (ab28812)
Ab28812, at a dilution of 1/50, staining ATF2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using the ATF2 antibody
Right image: using the same antibody preincubated with synthesized phospho peptide.
Western blot - ATF2 (phospho T71 or 53) antibody (ab28812)
All lanes : Anti-ATF2 (phospho T71 + T53) antibody (ab28812) at 1/1000 dilution
Lane 1 : untreated HeLa cells (5-30ug lysate).
Lane 2 : HeLa cells treated with UV light (5-30ug lysate).
Predicted band size : 52 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
References for Anti-ATF2 (phospho T71 + T53) antibody (ab28812)
This product has been referenced in:
- White SJ et al. Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress. J Cell Physiol 226:2841-8 (2011). WB.Read more (PubMed: 21302282) »
See 1 publication for this product
Publishing research using ab28812? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"