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Anti-ATF2 (phospho T71) antibody [E268] (ab32019)

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Overview

Product name

Anti-ATF2 (phospho T71) antibody [E268]
See all ATF2 products (33) ...

Description

Rabbit monoclonal [E268] to ATF2 (phospho T71)

Specificity

ab32019 only detects ATF2 phosphorylated on Threonine 71

Tested applications

WB, IP, ICC/IFmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic phospho peptide corresponding to residues surrounding Thr71 of human ATF2

Positive control

HeLa cells.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

SAPK and p38 MAPK activate, in response to cellular stress, ATF2 by phosphorylating the protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress induced transcription by ATF2.

Clonality

Monoclonal

Clone number

E268

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

  • Western blot - ATF2 (phospho T71) antibody [E268] (ab32019)Western blot - ATF2 (phospho T71) antibody [E268] (ab32019) image (enlarge)

  • Immunofluorescence - ATF2 (phospho T71) antibody [E268] (ab32019)Immunofluorescence - ATF2 (phospho T71) antibody [E268] (ab32019) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/100.

IP: 1/40.
WB: 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).

Is unsuitable for FACS or IHC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.

Tissue specificity

Abundant expression seen in the brain.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.

Post-translational
modifications

Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP15336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CREB 2 antibody
  • HB 16 antibody
  • Activating Transcription Factor 2 antibody
  • Activating Transcription Factor 2 antibody
  • ATF 2 antibody
  • Atf-2 antibody
  • Atf2 antibody
  • ATF2 protein antibody
  • ATF2_HUMAN antibody
  • cAMP Response Element Binding Protein 2 antibody
  • cAMP Response Element Binding Protein 2 antibody
  • cAMP response element binding protein CRE BP1 antibody
  • cAMP response element-binding protein CRE-BP1 antibody
  • cAMP-dependent transcription factor ATF-2 antibody
  • cAMP-responsive element-binding protein 2 antibody
  • CRE BP1 antibody
  • CRE-BP antibody
  • CREB 2 antibody
  • CREB-2 antibody
  • CREB2 antibody
  • CREB2 antibody
  • CREBP1 antibody
  • CREBP1 antibody
  • Cyclic AMP dependent transcription factor ATF 2 antibody
  • Cyclic AMP-dependent transcription factor ATF-2 antibody
  • Cyclic AMP-responsive element-binding protein 2 antibody
  • D130078H02Rik antibody
  • D18875 antibody
  • HB 16 antibody
  • HB16 antibody
  • HB16 antibody
  • MGC105211 antibody
  • MGC105222 antibody
  • MGC111558 antibody
  • MGC142504 antibody
  • mXBP antibody
  • mXBP antibody
  • MXBP protein antibody
  • Tg(Gzma-Klra1)7Wum antibody
  • TREB 7 antibody
  • TREB7 antibody
  • TREB7 antibody
see all

Database links

Anti-ATF2 (phospho T71) antibody [E268] images:

  Western blot - ATF2 (phospho T71) antibody [E268] (ab32019)

Western blot - ATF2 (phospho T71) antibody [E268] (ab32019)

All lanes : Anti-ATF2 (phospho T71) antibody [E268] (ab32019) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Anisomycin


Predicted band size : 54 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)

  Immunofluorescence - ATF2 (phospho T71) antibody [E268] (ab32019)

Immunofluorescence - ATF2 (phospho T71) antibody [E268] (ab32019)

ab32019 at a 1/100 dilution, staining human ATF2 in HeLa cells by Immunofluorescence

References for Anti-ATF2 (phospho T71) antibody [E268] (ab32019)

ab32019 has not yet been referenced specifically in any publications.

Publishing research using ab32019? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"