![Western blot - ATF3 antibody [44C3a] (ab58668) image](#ab58668.gif)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-ATF3 antibody [44C3a]
See all ATF3 products (4) ...
Description
Mouse monoclonal [44C3a] to ATF3
Tested applications
WB, Dot Blot, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment of Human ATF3
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab58668 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Clonality
Monoclonal
Clone number
44C3a
Isotype
IgG1
Research areas
Cancer >> Tumor biomarkers >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - ATF3 antibody [44C3a] (ab58668)
![Western blot - ATF3 antibody [44C3a] (ab58668) image](/ps/datasheet/Images/58/ab58668/ab58668.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 21 kDa).
Dot: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration. (PubMed: 20054152)
Target
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18847
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activating transcription factor 3 antibody
- ATF3 antibody
- ATF3_HUMAN antibody
see all
Database links
- Entrez Gene: 467 Human
- Omim: 603148 Human
- SwissProt: P18847 Human
- Unigene: 460 Human
Anti-ATF3 antibody [44C3a] images:
Western blot - ATF3 antibody [44C3a] (ab58668)
Anti-ATF3 antibody [44C3a] (ab58668) + immunising recombinant protein
Predicted band size : 21 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-ATF3 antibody [44C3a] (ab58668)
This product has been referenced in:
- Signorelli Set al. Differential effects of hypoxic stress in alveolar epithelial cells and microvascular endothelial cells. Cell Physiol Biochem 25:135-44 (2010). IHC-P; Human.Read more (PubMed: 20054152) »
- Jennings Pet al. Inter-laboratory comparison of human renal proximal tubule (HK-2) transcriptome alterations due to Cyclosporine A exposure and medium exhaustion. Toxicol In Vitro 23:486-99 (2009). IF; Human.Read more (PubMed: 19159671) »
See all 2 publications for this product
Publishing research using ab58668? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"