Anti-ATF3 antibody [44C3a] (ab58668)

Overview

• Product nameAnti-ATF3 antibody [44C3a]See all ATF3 primary antibodies ...
• Description
  Mouse monoclonal [44C3a] to ATF3
• Tested applicationsWB, Dot Blot, ICC/IF, IHC-Fr, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment of Human ATF3

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Purification notesab58668 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
• Clonality Monoclonal
• Clone number44C3a
• IsotypeIgG1
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P18847 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 467 Human
  • Omim: 603148 Human
  • SwissProt: P18847 Human
  • Unigene: 460 Human

• Alternative names
  Activating transcription factor 3 antibodyATF3 antibodyATF3_HUMAN antibody

  ATF3deltaZip2 antibodyATF3deltaZip2c antibodyATF3deltaZip3 antibodycAMP dependent transcription factor ATF3 antibodycAMP-dependent transcription factor ATF-3 antibodyCyclic AMP dependent transcription factor ATF3 antibodyCyclic AMP-dependent transcription factor ATF-3 antibody

  see all

Anti-ATF3 antibody [44C3a] images

• 

  Western blot - ATF3 antibody [44C3a] (ab58668)
  Anti-ATF3 antibody [44C3a] (ab58668) + immunising recombinant protein
  
  Predicted band size : 21 kDa
  Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody [44C3a] (ab58668)

  ab58668 staining ATF3 in A549 cells treated with ionomycin Ca²⁺ salt (ab120116), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin Ca²⁺ salt, as described in literature.
  The cells were incubated at 37°C for 2h in media containing different concentrations of ab120116 (ionomycin Ca²⁺ salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody [44C3a] (ab58668)
  ab58668 staining ATF3 in A549 cells treated with ionomycin (free acid) (ab120370), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin (free acid), as described in literature.
  The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370 (Ionomycin (free acid)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  
• 

  Immunocytochemistry/ Immunofluorescence-Anti-ATF3 antibody [44C3a](ab58668)
  ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin (ab120286), by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.
  The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286 (thapsigargin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.