ATF3 antibody [44C3a] (ab58668)

Product Name 

ATF3 antibody [44C3a]

Product type 

Primary antibodies

Description 

Mouse monoclonal [44C3a] to ATF3

Immunogen 

Recombinant fragment of Human ATF3

Reacts with 
(species key)

Hu

Tested applications 
(see key)

Dot, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 21 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cancer >> Tumor biomarkers >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA. In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. Three transcript variants encoding two different isoforms have been found for this gene.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

44C3a

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Purification notes 

ab58668 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for ATF3 antibody [44C3a] images, references, products related to ab58668 and other tools.

ATF3 antibody [44C3a] images:

References for ATF3 antibody [44C3a] (ab58668)

  This product has been used in:

Jennings P et al. Inter-laboratory comparison of human renal proximal tubule (HK-2) transcriptome alterations due to Cyclosporine A exposure and medium exhaustion. Toxicol In Vitro 23:486-99 (2009). IF; Human. PubMed: 19159671

If you publish research using ab58668 please let us know so that we can cite the reference on this datasheet.

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