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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-ATF3 antibody [44C3a]
See all ATF3 products (4) ...
Mouse monoclonal [44C3a] to ATF3
WB, Dot Blot, IHC-Pmore details
Reacts with
Human
Recombinant fragment of Human ATF3
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
Concentration information loading...
Protein G purified
ab58668 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Monoclonal
44C3a
IgG1
Cancer >> Tumor biomarkers >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Western blot - ATF3 antibody [44C3a] (ab58668)
(enlarge)
Our Abpromise guarantee covers the use of ab58668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 21 kDa).
Dot: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration. (PubMed: 20054152)
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Nucleus.
Target information above from: UniProt accessionP18847
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ATF3 antibody [44C3a] (ab58668)
![Western blot - ATF3 antibody [44C3a] (ab58668)](/ps/datasheet/Images/58/ab58668/ab58668.gif)
Anti-ATF3 antibody [44C3a] (ab58668) + immunising recombinant protein
Predicted band size : 21 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab58668? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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![Western blot - ATF3 antibody [44C3a] (ab58668)](/ps/datasheet/Images/58/ab58668/ab58668.gif)
Anti-ATF3 antibody [44C3a] (ab58668) + immunising recombinant protein
Predicted band size : 21 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
0
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