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Overview

  • Product nameAnti-ATF3 antibody [44C3a]
    See all ATF3 primary antibodies
  • Description
    Mouse monoclonal [44C3a] to ATF3
  • Tested applicationsWB, Dot Blot, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment of Human ATF3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab58668 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ClonalityMonoclonal
  • Clone number44C3a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 21 kDa).
Dot Blot Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20054152

Target

  • FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
  • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 3 antibody
    • ATF3 antibody
    • ATF3_HUMAN antibody
    • ATF3deltaZip2 antibody
    • ATF3deltaZip2c antibody
    • ATF3deltaZip3 antibody
    • cAMP dependent transcription factor ATF3 antibody
    • cAMP-dependent transcription factor ATF-3 antibody
    • Cyclic AMP dependent transcription factor ATF3 antibody
    • Cyclic AMP-dependent transcription factor ATF-3 antibody
    see all

Anti-ATF3 antibody [44C3a] images

  • Anti-ATF3 antibody [44C3a] (ab58668) + immunising recombinant protein

    Predicted band size : 21 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab58668 staining ATF3 in A549 cells treated with ionomycin Ca2+ salt (ab120116), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin Ca2+ salt, as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120116 (ionomycin Ca2+ salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • ab58668 staining ATF3 in A549 cells treated with ionomycin (free acid) (ab120370), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin (free acid), as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370 (Ionomycin (free acid)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin (ab120286), by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286 (thapsigargin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References for Anti-ATF3 antibody [44C3a] (ab58668)

This product has been referenced in:
  • Nascimento DS  et al. Satellite Glial Cells Surrounding Primary Afferent Neurons Are Activated and Proliferate during Monoarthritis in Rats: Is There a Role for ATF3? PLoS One 9:e108152 (2014). IHC-FoFr ; Rat . Read more (PubMed: 25247596) »
  • Signorelli S  et al. Differential effects of hypoxic stress in alveolar epithelial cells and microvascular endothelial cells. Cell Physiol Biochem 25:135-44 (2010). IHC-P ; Human . Read more (PubMed: 20054152) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Dorsal Root Ganglia Frozen Sections)
Antigen retrieval step None
Permeabilization Yes - Triton X-100
Specification Dorsal Root Ganglia Frozen Sections
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Jean-Marc Guedon

Verified customer

Submitted Dec 01 2015

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain (Locus coeruleus))
Permeabilization Yes - 0.1%triton+0.6%H2O2 in 1XTBST
Specification Brain (Locus coeruleus)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 30 2015

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Injured spinal cord)
Specification Injured spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"