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Western blot - ATF3 antibody (ab70005)
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Product Name
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ATF3 antibody
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See all ATF3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATF3
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Immunogen
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Synthetic peptide derived from internal region of human ATF3.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects endogenous levels of total ATF3 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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RAW264.7 cell extract
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Tumor biomarkers >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA. In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. Three transcript variants encoding two different isoforms have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATF3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATF3 antibody images, references, products related to ab70005 and other tools.
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ATF3 antibody images:
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 Western blot - ATF3 antibody (ab70005)
All lanes : ATF3 antibody (ab70005) at 1/500 dilution
Lane 1 : RAW264.7 cell extract
Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 21 kDa
Observed band size : 21 kDa
Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to ATF3
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ATF3 antibody - more information
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Customer reviews (feedback) regarding ATF3 antibody |
Customer FAQs regarding ATF3 antibody |
Protocols for ATF3 antibody |
Price and availability of products related to ATF3 antibody |
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ATF3 antibody - related products:
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ATF3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ATF3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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