Anti-ATF3 antibody (ab70005)

Overview

• Product nameAnti-ATF3 antibodySee all ATF3 primary antibodies ...
• Description
  Rabbit polyclonal to ATF3
• SpecificityDetects endogenous levels of total ATF3 protein.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthetic peptide derived from internal region of human ATF3.

• Positive controlRAW264.7 cell extract

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P18847 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 467 Human
  • Entrez Gene: 11910 Mouse
  • Entrez Gene: 25389 Rat
  • Omim: 603148 Human
  • SwissProt: P18847 Human
  • SwissProt: Q60765 Mouse
  • SwissProt: P29596 Rat
  • Unigene: 460 Human

  • Unigene: 2706 Mouse
  • Unigene: 9664 Rat

  see all

• Alternative names
  Activating transcription factor 3 antibodyATF3 antibodyATF3_HUMAN antibody

  ATF3deltaZip2 antibodyATF3deltaZip2c antibodyATF3deltaZip3 antibodycAMP dependent transcription factor ATF3 antibodycAMP-dependent transcription factor ATF-3 antibodyCyclic AMP dependent transcription factor ATF3 antibodyCyclic AMP-dependent transcription factor ATF-3 antibody

  see all

Anti-ATF3 antibody images

• 

  Western blot - ATF3 antibody (ab70005)
  All lanes : Anti-ATF3 antibody (ab70005) at 1/500 dilution
  
  Lane 1 : RAW264.7 cell extract
  Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 21 kDa
  Observed band size : 21 kDa
  Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.