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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-ATF3 antibody
See all ATF3 products (4) ...
Rabbit polyclonal to ATF3
Detects endogenous levels of total ATF3 protein.
WB, ELISAmore details
Reacts with
Mouse, Rat, Human
Synthetic peptide derived from internal region of human ATF3.
RAW264.7 cell extract
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Polyclonal
IgG
Cancer >> Tumor biomarkers >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Western blot - ATF3 antibody (ab70005)
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Our Abpromise guarantee covers the use of ab70005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA: 1/5000
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Nucleus.
Target information above from: UniProt accessionP18847
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ATF3 antibody (ab70005)

All lanes : Anti-ATF3 antibody (ab70005) at 1/500 dilution
Lane 1 : RAW264.7 cell extract
Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 21 kDa
Observed band size : 21 kDa
Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.
ab70005 has not yet been referenced specifically in any publications.
Publishing research using ab70005? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-ATF3 antibody (ab70005) at 1/500 dilution
Lane 1 : RAW264.7 cell extract
Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 21 kDa
Observed band size : 21 kDa
Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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