Overview
- Product nameAnti-ATF3 antibodySee all ATF3 primary antibodies ...
- DescriptionRabbit polyclonal to ATF3
- SpecificityDetects endogenous levels of total ATF3 protein.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat - Immunogen
Synthetic peptide derived from internal region of human ATF3.
- Positive controlRAW264.7 cell extract
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab70005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
| ELISA | ELISA: 1/5000. |
Target
- FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
- Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 467 Human
- Entrez Gene: 11910 Mouse
- Entrez Gene: 25389 Rat
- Omim: 603148 Human
- SwissProt: P18847 Human
- SwissProt: Q60765 Mouse
- SwissProt: P29596 Rat
- Unigene: 460 Human
- Unigene: 2706 Mouse
- Unigene: 9664 Rat
see all
Target information above from: UniProt accession
P18847
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Activating transcription factor 3 antibodyATF3 antibodyATF3_HUMAN antibody
- ATF3deltaZip2 antibodyATF3deltaZip2c antibodyATF3deltaZip3 antibodycAMP dependent transcription factor ATF3 antibodycAMP-dependent transcription factor ATF-3 antibodyCyclic AMP dependent transcription factor ATF3 antibodyCyclic AMP-dependent transcription factor ATF-3 antibody
see all
Anti-ATF3 antibody images
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All lanes : Anti-ATF3 antibody (ab70005) at 1/500 dilution
Lane 1 : RAW264.7 cell extract
Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 21 kDa
Observed band size : 21 kDa
Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ATF3 antibody (ab70005)
ab70005 has not yet been referenced specifically in any publications.
