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Anti-ATF3 antibody (ab70005)

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Overview

Product name

Anti-ATF3 antibody
See all ATF3 products (4) ...

Description

Rabbit polyclonal to ATF3

Specificity

Detects endogenous levels of total ATF3 protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide derived from internal region of human ATF3.

Positive control

RAW264.7 cell extract

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Tumor biomarkers >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - ATF3 antibody (ab70005)Western blot - ATF3 antibody (ab70005) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab70005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Function

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP18847 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Activating transcription factor 3 antibody
  • ATF3 antibody
  • ATF3_HUMAN antibody
  • ATF3deltaZip2 antibody
  • ATF3deltaZip2c antibody
  • ATF3deltaZip3 antibody
  • cAMP dependent transcription factor ATF3 antibody
  • cAMP-dependent transcription factor ATF-3 antibody
  • Cyclic AMP dependent transcription factor ATF3 antibody
  • Cyclic AMP-dependent transcription factor ATF-3 antibody
see all

Database links

see all

Anti-ATF3 antibody images:

  Western blot - ATF3 antibody (ab70005)

Western blot - ATF3 antibody (ab70005)

All lanes : Anti-ATF3 antibody (ab70005) at 1/500 dilution

Lane 1 : RAW264.7 cell extract
Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 21 kDa
Observed band size : 21 kDa
Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATF3 antibody (ab70005)

ab70005 has not yet been referenced specifically in any publications.

Publishing research using ab70005? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"