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Western blot - ATF3 antibody (ab87213)
(enlarge)
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Product Name
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ATF3 antibody
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See all ATF3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATF3
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Immunogen
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Synthetic peptide corresponding to a region within internal amino acids 72 - 121 (LGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK of Human ATF3 (NP_001665)
BLAST 'LGVSITKAEV APEEDERKKR RRERNKIAAA KCRNKKKEKT ECLQKESEKL ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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OVCAR-3 cell lysate
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Tumor biomarkers >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA. In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. Three transcript variants encoding two different isoforms have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATF3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATF3 antibody images, references, products related to ab87213 and other tools.
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ATF3 antibody images:
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 Western blot - ATF3 antibody (ab87213)
ATF3 antibody (ab87213) at 1 µg/ml (in 5% skim milk / PBS buffer) + OVCAR-3 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 21 kDa
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to ATF3
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ATF3 antibody - more information
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Customer reviews (feedback) regarding ATF3 antibody |
Customer FAQs regarding ATF3 antibody |
Protocols for ATF3 antibody |
Price and availability of products related to ATF3 antibody |
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ATF3 antibody - related products:
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ATF3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ATF3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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