Anti-ATF3 antibody (ab87213)

Overview

• Product nameAnti-ATF3 antibodySee all ATF3 primary antibodies ...
• Description
  Rabbit polyclonal to ATF3
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
• Immunogen

  Synthetic peptide corresponding to a region within internal amino acids 72 - 121 (LGVSITKAEVAPEEDERKKRRRERNKIAAAKCRNKKKEK TECLQKESEKL) of Human ATF3 (NP_001665)

  Run BLAST with Run BLAST with
• Positive controlOVCAR-3 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P18847 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 467 Human
  • Entrez Gene: 11910 Mouse
  • Entrez Gene: 25389 Rat
  • Omim: 603148 Human
  • SwissProt: P18847 Human
  • SwissProt: Q60765 Mouse
  • SwissProt: P29596 Rat
  • Unigene: 460 Human

  • Unigene: 2706 Mouse
  • Unigene: 9664 Rat

  see all

• Alternative names
  Activating transcription factor 3 antibodyATF3 antibodyATF3_HUMAN antibody

  ATF3deltaZip2 antibodyATF3deltaZip2c antibodyATF3deltaZip3 antibodycAMP dependent transcription factor ATF3 antibodycAMP-dependent transcription factor ATF-3 antibodyCyclic AMP dependent transcription factor ATF3 antibodyCyclic AMP-dependent transcription factor ATF-3 antibody

  see all

Anti-ATF3 antibody images

• 

  Western blot - ATF3 antibody (ab87213)
  Anti-ATF3 antibody (ab87213) at 1 µg/ml (in 5% skim milk / PBS buffer) + OVCAR-3 cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 21 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody (ab87213)

  ab87213 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87213 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.