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Overview

  • Product nameAnti-ATF3 antibodySee all ATF3 primary antibodies ...
  • Description
    Rabbit polyclonal to ATF3
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 72 - 121 (LGVSITKAEVAPEEDERKKRRRERNKIAAAKCRNKKKEK TECLQKESEKL) of Human ATF3 (NP_001665)

  • Positive controlOVCAR-3 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab87213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.

Target

  • Alternative names
      Activating transcription factor 3 antibodyATF3 antibodyATF3_HUMAN antibody
      ATF3deltaZip2 antibodyATF3deltaZip2c antibodyATF3deltaZip3 antibodycAMP dependent transcription factor ATF3 antibodycAMP-dependent transcription factor ATF-3 antibodyCyclic AMP dependent transcription factor ATF3 antibodyCyclic AMP-dependent transcription factor ATF-3 antibody
    see all

Anti-ATF3 antibody images

  • Anti-ATF3 antibody (ab87213) at 1 µg/ml (in 5% skim milk / PBS buffer) + OVCAR-3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa
  • ab87213 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87213 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ATF3 antibody (ab87213)

ab87213 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"