Anti-ATF4 antibody (ab23760)

Overview

• Product nameAnti-ATF4 antibodySee all ATF4 primary antibodies ...
• Description
  Rabbit polyclonal to ATF4
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human ATF4.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab24034.)

• Positive controlATF4 tagged recombinant protein This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

Applications

Target

• FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
• Sequence similaritiesBelongs to the bZIP family.
  Contains 1 bZIP domain.
• Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.

Target information above from: UniProt accession P18848 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 468 Human
  • Entrez Gene: 11911 Mouse
  • Entrez Gene: 79255 Rat
  • NCBI: 33469976 Human
  • Omim: 604064 Human
  • SwissProt: Q96AQ3 Human
  • SwissProt: P18848 Human
  • SwissProt: Q5U4B2 Mouse

  • SwissProt: Q8CF69 Mouse
  • SwissProt: Q06507 Mouse
  • SwissProt: Q9ES19 Rat
  • Unigene: 496487 Human
  • Unigene: 641 Mouse
  • Unigene: 2423 Rat

  see all

• Alternative names
  Activating Transcription Factor 4 antibodyATF 4 antibodyATF4 antibody

  ATF4 protein antibodyATF4_HUMAN antibodycAMP-dependent transcription factor ATF-4 antibodycAMP-responsive element-binding protein 2 antibodyCREB 2 antibodyCREB-2 antibodyCREB2 antibodyCyclic AMP dependent transcription factor ATF 4 antibodyCyclic AMP response element binding protein 2 antibodyCyclic AMP response element binding protein 2 antibodyCyclic AMP-dependent transcription factor ATF-4 antibodyCyclic AMP-responsive element-binding protein 2 antibodyDNA binding protein TAXREB67 antibodyDNA binding protein TAXREB67 antibodyDNA-binding protein TAXREB67 antibodyTax Responsive Enhancer Element B67 antibodyTax-responsive enhancer element-binding protein 67 antibodyTAXREB67 antibodyTXREB antibody

  see all

Anti-ATF4 antibody images

• 

  Western blot - ATF4 antibody (ab23760)
  Anti-ATF4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µg
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 39 kDa
  Observed band size : 76 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATF4 antibody (ab23760)

  ICC/IF image of ab23760 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23760 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.