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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Anti-ATF6 antibody [70B1413] - ChIP Grade (ab11909)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab11909? Please let us know so that we can cite the reference in this datasheet
ab11909 has been referenced in 1 publications.
- Ou YC et al. Indomethacin induces apoptosis in 786-O renal cell carcinoma cells by activating mitogen-activated protein kinases and AKT. Eur J Pharmacol 563:49-60 (2007). PubMed: 17341418
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![- ATF6 antibody [70B1413] - ChIP Grade (ab11909)](/ps/datasheet/Images/11/ab11909/11909_2.jpg)
- ATF6 antibody [70B1413] - ChIP Grade (ab11909)
Ab11909 (3ug/ml) staining of mouse ATF6 in NIH3T3 cell lysate by Western Blot. The ~36 kDa observed band has not been characterized; it may be an ATF6 breakdown/cleavage product.
![Immunocytochemistry/ Immunofluorescence-ATF6 antibody [70B1413] - ChIP Grade(ab11909)](/ps/datasheet/images/11/ab11909/ATF6-Primary-antibodies-ab11909-1.jpg)
Immunocytochemistry/ Immunofluorescence-ATF6 antibody [70B1413] - ChIP Grade(ab11909)
ICC/IF image of ab11909 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11909, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry-Anti-ATF6 antibody [70B1413] - ChIP Grade(ab11909)](/ps/datasheet/images/11/ab11909/ATF6-Primary-antibodies-ab11909-2.jpg)
Flow Cytometry-Anti-ATF6 antibody [70B1413] - ChIP Grade(ab11909)
Overlay histogram showing HeLa cells stained with ab11909 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11909, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
![ATF6 antibody [70B1413] for WB in Human (11909)](/ps/Reviews/Images/ab7882_33874.jpg)
ATF6 antibody [70B1413] for WB in Human (11909)
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