ATF6 antibody [70B1413] - ChIP Grade (ab11909)

Product Name 

ATF6 antibody [70B1413] - ChIP Grade

Product type 

Primary antibodies

Description 

Mouse monoclonal [70B1413] to ATF6 - ChIP Grade

Immunogen 

Full length protein (Human).

Reacts with 
(species key)

Hu, Ms, Rat, Hm, Rb

Specificity 

This antibody only recognizes the 90kDa form of ATF6.

Tested applications 
(see key)

ChIP, ICC/IF, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 75 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293 whole cell extract.

Cellular localization 

Endoplasmic reticulum: Type II membrane protein in the endoplasmic reticulum. Under ER stress the cleaved N terminal cytoplasmic domain translocates into the nucleus.

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

Relevance 

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It also interacts with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site 1 and site 2 proteases. ATF6 is N glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

70B1413

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for ATF6 antibody [70B1413] - ChIP Grade images, references, products related to ab11909 and other tools.

ATF6 antibody [70B1413] - ChIP Grade images:

References for ATF6 antibody [70B1413] - ChIP Grade (ab11909)

  This product has been used in:

Ou YC et al. Indomethacin induces apoptosis in 786-O renal cell carcinoma cells by activating mitogen-activated protein kinases and AKT. Eur J Pharmacol 563:49-60 (2007). PubMed: 17341418

If you publish research using ab11909 please let us know so that we can cite the reference on this datasheet.

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