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- Proteins and Peptides
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Product name
Anti-ATF6 antibody
See all ATF6 products (8) ...
Description
Rabbit polyclonal to ATF6
Tested applications
ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: PYLRDQQRNQTNT, corresponding to amino acids 635-647 of Human ATF6.
PYLRDQQRNQTNT
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Glycerol
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/100 - 1/1000.ELISA: 1/100 - 1/1000.
Target
Function
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Domain
The basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
Post-translational
modifications
During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK.
Cellular localization
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
Target information above from: UniProt accessionP18850
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
see all
Database links
- Entrez Gene: 22926 Human
- Omim: 605537 Human
- SwissProt: P18850 Human
- Unigene: 433046 Human
- Unigene: 492740 Human
References for Anti-ATF6 antibody (ab22341)
This product has been referenced in:
- Rouschop KM et al. The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5. J Clin Invest 120:127-41 (2010).Read more (PubMed: 20038797) »
See 1 publication for this product
Publishing research using ab22341? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"