Anti-ATF6 antibody (ab37149)

Western blot - ATF6 antibody (ab37149)

Lane 1 : Anti-ATF6 antibody (ab37149) at 0.5 µg/ml
Lane 2 : Anti-ATF6 antibody (ab37149) at 1 µg/ml

Lane 1 : MDA MB 361 cell lysate
Lane 2 : MDA MB 361 cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
goat anti-rabbit HRP at 1/10000 dilution

Predicted band size : 75 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)

Immunocytochemistry/ Immunofluorescence - ATF6 antibody (ab37149)

ab37149 staining human Hella cells by ICC/IF. Cells were PFA fixed and permeabilized for 10 minutes in 0.1% Triton X-100 prior to blocking in 3% BSA for 15 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. The secondary antibody used was Alexa Fluor®568 goat anti-rabbit diluted at 1/1000.

This image is courtesy of an Abreview submitted by Dr Dan Tattersall

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ATF6 antibody(ab37149)

Ab37149 staining human normal pancreas. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.