Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-ATF6 antibody
See all ATF6 products (8) ...
Description
Rabbit polyclonal to ATF6
Specificity
Based on 100% sequence alignment, this antibody detects ATF6 isoform A (alpha). The antibody is predicted to recognize the 50kDa fragment as the it recognizes a peptide sequence from near the amino terminus. We have not tested stressed cells to see if the 50kDa cleavage product can be detected.
Tested applications
ICC, ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to 17 amino acids near to the N terminus of the human ATF6 protein.
Positive control
MDA MB 361 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Immunoaffinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependent concentration.
ICC/IF: Use a concentration of 10 µg/ml
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).
Target
Function
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Domain
The basic domain functions as a nuclear localization signal.
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
Post-translational
modifications
During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
Phosphorylated in vitro by MAPK14/P38MAPK.
Cellular localization
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
Target information above from: UniProt accessionP18850
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activating transcription factor 6 alpha antibody
- Activating transcription factor 6 antibody
- ATF 6 antibody
see all
Database links
- Entrez Gene: 22926 Human
- Entrez Gene: 226641 Mouse
- Omim: 605537 Human
- SwissProt: P18850 Human
- Unigene: 433046 Human
- Unigene: 492740 Human
Anti-ATF6 antibody images:
Western blot - ATF6 antibody (ab37149)
Lane 1 : Anti-ATF6 antibody (ab37149) at 0.5 µg/ml
Lane 2 : Anti-ATF6 antibody (ab37149) at 1 µg/ml
Lane 1 : MDA MB 361 cell lysate
Lane 2 : MDA MB 361 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
goat anti-rabbit HRP at 1/10000 dilution
Predicted band size : 75 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - ATF6 antibody (ab37149)
ab37149 staining human Hella cells by ICC/IF. Cells were PFA fixed and permeabilized for 10 minutes in 0.1% Triton X-100 prior to blocking in 3% BSA for 15 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. The secondary antibody used was Alexa Fluor®568 goat anti-rabbit diluted at 1/1000.
This image is courtesy of an Abreview submitted by Dr Dan Tattersall
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ATF6 antibody(ab37149)
Ab37149 staining human normal pancreas. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-ATF6 antibody (ab37149)
This product has been referenced in:
- Guan Met al. Nelfinavir induces liposarcoma apoptosis through inhibition of regulated intramembrane proteolysis of SREBP-1 and ATF6. Clin Cancer Res 17:1796-806 (2011). WB; Human.Read more (PubMed: 21355074) »
- Shirley CMet al. Bortezomib induction of C/EBP{beta} mediates Epstein-Barr virus lytic activation in Burkitt lymphoma. Blood 117:6297-303 (2011).Read more (PubMed: 21447826) »
See all 7 publications for this product
Publishing research using ab37149? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"