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Overview

  • Product nameATF6 protein (Tagged-His Tag)See all ATF6 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 567-670 of Human ATF6 with N terminal His tag, 104aa, 25kDa, Uniprot ID = P18850.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:reconstitution with 92 µl aqua dest.
  • Storage instructionsStore at 4°C. Upon reconstitution store at -80ºC.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesYVVSFRRDHLLLPATTHNKTTRPKMSIVLPAININENVIN GQDYEVMMQIDCQ VMDTRILHIKSSSVPPYLRDQQRNQ TNTFFGSPPAATEATHVVSTIPESLQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92267 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Activating transcription factor 6Activating transcription factor 6 alphaATF 6
        ATF6ATF6 alphaATF6-alphaATF6AATF6A_HUMANcAMP dependent transcription factor ATF 6 alphacAMP-dependent transcription factor ATF-6 alphaCyclic AMP dependent transcription factor ATF 6 alphaDKFZp686P2194ESTM49FLJ21663Processed cyclic AMP dependent transcription factor ATF 6 alphaProcessed cyclic AMP-dependent transcription factor ATF-6 alpha
      see all
  • FunctionTranscription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • DomainThe basic domain functions as a nuclear localization signal.
    The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
  • Post-translational
    modifications    During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
    N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
    Phosphorylated in vitro by MAPK14/P38MAPK.
  • Cellular localizationEndoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.

    • Target information above from: UniProt accession P18850 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ATF6 protein (Tagged-His Tag) images

    • SDS-PAGE - ATF6 protein (Tagged-His Tag) (ab92267)
      SDS-PAGE - ATF6 protein (Tagged-His Tag) (ab92267)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ATF6 protein (Tagged-His Tag) (ab92267)

    ab92267 has not yet been referenced specifically in any publications.

    Publishing research using ab92267 ? Please let us know so that we can cite the reference in this datasheet

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