Anti-ATG16L1 antibody (ab188642)
Key features and details
- Rabbit polyclonal to ATG16L1
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ATG16L1 antibody
See all ATG16L1 primary antibodies -
Description
Rabbit polyclonal to ATG16L1 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ATG16L1 (internal sequence). 18 amino acids.
Database link: Q676U5 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 2 - 4 µg/ml.
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WB |
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 68 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 2 - 4 µg/ml. |
WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 68 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. -
Involvement in disease
Inflammatory bowel disease 10 -
Sequence similarities
Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats. -
Post-translational
modificationsProteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli. -
Cellular localization
Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. - Information by UniProt
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Database links
- Entrez Gene: 55054 Human
- Entrez Gene: 77040 Mouse
- Entrez Gene: 363278 Rat
- Omim: 610767 Human
- SwissProt: Q676U5 Human
- SwissProt: Q8C0J2 Mouse
- Unigene: 529322 Human
- Unigene: 272972 Mouse
see all -
Form
There are 4 isoforms produced by alternative splicing. -
Alternative names
- A16L1_HUMAN antibody
- APG16 like 1 antibody
- APG16-like 1 antibody
see all
Images
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Lane 1 : Anti-ATG16L1 antibody (ab188642) at 0.25 µg/ml
Lane 2 : Anti-ATG16L1 antibody (ab188642) at 0.5 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 68 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue sections labeling ATG16L1 using ab188642 at 5 µg/ml.
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Immunocytochemical/ Immunofluorescence analysis of HeLa cells labeling ATG16L1 using ab188642 at 4 µg/ml.
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Immunocytochemical analysis of HeLa cells labeling ATG16L1 using ab188642 at 2 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab188642 has been referenced in 7 publications.
- Zhao J et al. miR-130a-3p, a Preclinical Therapeutic Target for Crohn's Disease. J Crohns Colitis 15:647-664 (2021). PubMed: 33022049
- He Z et al. MicroRNA 223 Targeting ATG16L1 Affects Microglial Autophagy in the Kainic Acid Model of Temporal Lobe Epilepsy. Front Neurol 12:704550 (2021). PubMed: 34381417
- Ou M et al. Emerging roles of let-7d in attenuating pulmonary arterial hypertension via suppression of pulmonary artery endothelial cell autophagy and endothelin synthesis through ATG16L1 downregulation. Int J Mol Med 46:83-96 (2020). PubMed: 32319531
- Liu K et al. MicroRNA-106a Inhibits Autophagy Process and Antimicrobial Responses by Targeting ULK1, ATG7, and ATG16L1 During Mycobacterial Infection. Front Immunol 11:610021 (2020). PubMed: 33505399
- Pellegrini D et al. Quantitative Microproteomics Based Characterization of the Central and Peripheral Nervous System of a Mouse Model of Krabbe Disease. Mol Cell Proteomics 18:1227-1241 (2019). PubMed: 30926673
- Zhang K et al. PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib. Cell Death Dis 9:312 (2018). PubMed: 29472524
- Liu H et al. Oxidative Stress Mediates Microcystin-LR-Induced Endoplasmic Reticulum Stress and Autophagy in KK-1 Cells and C57BL/6 Mice Ovaries. Front Physiol 9:1058 (2018). PubMed: 30131715