Anti-ATG2B antibody - N-terminal (ab189934)
Key features and details
- Rabbit polyclonal to ATG2B - N-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG2B antibody - N-terminal
See all ATG2B primary antibodies -
Description
Rabbit polyclonal to ATG2B - N-terminal -
Host species
Rabbit -
Specificity
ab189934 is predicted to not cross-react with other ATG2A. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATG2B (N terminal). The peptide corresponds to 19 amino acids. (NP_060506)
Database link: Q96BY7 -
Positive control
- K562 cell lysate; Human colon tissue; K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 233 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 233 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Sequence similarities
Belongs to the ATG2 family. - Information by UniProt
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Database links
- Entrez Gene: 55102 Human
- SwissProt: Q96BY7 Human
- Unigene: 168241 Human
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Alternative names
- 2410024A21Rik antibody
- AI047755 antibody
- AI503411 antibody
see all
Images
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Lane 1 : Anti-ATG2B antibody - N-terminal (ab189934) at 1 µg/ml
Lane 2 : Anti-ATG2B antibody - N-terminal (ab189934) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 233 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling ATG2B with ab189934 at 5 µg/ml.
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Immunofluorescent analysis of K562 cells labeling ATG2B with ab189934 at 20 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab189934 has been referenced in 4 publications.
- Zhang J et al. LncRNA-HOTAIR activates autophagy and promotes the imatinib resistance of gastrointestinal stromal tumor cells through a mechanism involving the miR-130a/ATG2B pathway. Cell Death Dis 12:367 (2021). PubMed: 33824300
- Li Y et al. The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide-induced acute lung injury through the miR-17-5p/ATG2/ATG7/ATG16 axis. J Cell Mol Med 25:8062-8073 (2021). PubMed: 34180119
- Bi X et al. Crocin exerts anti-proliferative and apoptotic effects on cutaneous squamous cell carcinoma via miR-320a/ATG2B. Bioengineered 12:4569-4580 (2021). PubMed: 34320900
- Zhang W et al. MiR-143-3p targets ATG2B to inhibit autophagy and promote endothelial progenitor cells tube formation in deep vein thrombosis. Tissue Cell 67:101453 (2020). PubMed: 33130456