Overview
- Product nameAnti-ATG4D antibodySee all ATG4D primary antibodies ...
- DescriptionSheep polyclonal to ATG4D
- Tested applicationsWB, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Human
Predicted to work with: Monkey - Immunogen
Synthetic peptide from human ATG4D, conjugated to an immunogenic carrier protein.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, Whole serum - PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab64735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/200 - 1/3000. Predicted molecular weight: 53 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
| IHC-P | IHC-P: 1/200 - 1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IHC-Fr | IHC-Fr: 1/200 - 1/3000. |
Target
- FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
- Tissue specificityMainly expressed in skeletal muscle and, to a lower extent, in testis.
- Sequence similaritiesBelongs to the peptidase C54 family.
- Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 84971 Human
- Entrez Gene: 84971 Human
- Omim: 611340 Human
- SwissProt: Q86TL0 Human
- SwissProt: Q86TL0 Human
- Unigene: 512799 Human
Target information above from: UniProt accession
Q86TL0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 9830134P12Rik antibodyAPG4 D antibodyAPG4D antibody
- ATG4 autophagy related 4 homolog D (S. cerevisiae) antibodyAtg4d antibodyATG4D_HUMAN antibodyAtg4dl antibodyAUT like 4 cysteine endopeptidase antibodyAUT-like 4 cysteine endopeptidase antibodyAUTL4 antibodyAutophagin 4 antibodyAutophagin-4 antibodyAutophagy related cysteine endopeptidase 4 antibodyAutophagy related protein 4 homolog D antibodyAutophagy-related cysteine endopeptidase 4 antibodyAutophagy-related protein 4 homolog D antibodyCysteine protease ATG4D antibodyCysteine protease involved in autophagy antibodyMGC31226 antibody
see all
References for Anti-ATG4D antibody (ab64735)
ab64735 has not yet been referenced specifically in any publications.
