Anti-ATG8 antibody (ab4753)

Buenas Tardes:
Un cliente está usando el Anti-ATG8 antibody (ab4753) para reconocer ATG-8 de Trichomonas vaginalis y han obtenido una banda de aprox. 31 KDa sin obtener la banda esperada de 14 KDa.
Ustedes nos podrían dar algún dato, con respecto al reconocimiento de mayor peso molecular que nos está dando.
Gracias.

ANSWER:

Gracias por contactarnos.

Para tratar de entender mejor el motivo por el que observan una banda a un mayor peso molecular que el esperado, le pediría por favor que nos rellene el cuestionario adjunto al mail, en el que se piden detalles acerca de las condiciones de uso del anticuerpo, así como datos acerca del lote y el pedido.

Esta información nos permite monitorizar la calidad de nuestros productos, así como ofrecerle sugerencias (en caso de haberlas) susceptibles de mejorar el protocolo seguido.

En caso de no tener ninguna sugerencia, estando el producto en garantía, estaremos encantados de ofrecerle un reemplazo o reembolso.

Si pudiera adjuntar imágenes al protocolo también se lo agradecería.

Quedo pues a la espera de su respuesta para darle una solución adecuada.

I would like to test ab4753 with C. neoformans.

ANSWER:

I am very pleased to hear you would like to accept our offer and test ab4753 in C. neoformans. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forC. neoformansand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

What is the sequence of the peptide used to generate this antibody? Just wanted to check the homology between this sequence and proteins in other species; we just placed an order for this product.

ANSWER:

Thank you for your enquiry.

Development of the antigen for antibody production is a confidential/proprietary process which we do not have authority (or knowledge) to detail at this time.

Our apologies.