Anti-ATL3 antibody (ab117834)

Overview

• Product nameAnti-ATL3 antibodySee all ATL3 primary antibodies ...
• Description
  Rabbit polyclonal to ATL3
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide, corresponding to a region within amino acids 491-541 of Human ATL3 (NP_056274.3).

• Positive controlHeLa, Jurkat, 293T and Mouse NIH3T3 whole cell lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium azide
  Constituents: 99% Tris buffered saline, 0.1% BSA
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab117834 was affinity purified using an epitope specific to ATL3 immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Second Messenger
  • Nucleotide Messenger
  • GTP

Applications

Target

• FunctionGTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.
• Tissue specificityExpressed in peripheral tissues (at protein level).
• Sequence similaritiesBelongs to the GBP family. Atlastin subfamily.
• Cellular localizationEndoplasmic reticulum membrane.

Target information above from: UniProt accession Q6DD88 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 25923 Human
  • Entrez Gene: 25923 Human
  • Entrez Gene: 109168 Mouse
  • Omim: 609369 Human
  • SwissProt: Q6DD88 Human
  • SwissProt: Q6DD88 Human
  • SwissProt: Q91YH5 Mouse
  • Unigene: 356719 Human

  • Unigene: 264016 Mouse

  see all

• Alternative names
  4633402C03Rik antibody5730596K20Rik antibodyAI465397 antibody

  ATL3 antibodyATLA3_HUMAN antibodyAtlastin 3 like antibodyAtlastin GTPase 3 antibodyAtlastin-3 antibodyAW228836 antibodyDKFZp564J0863 antibodyMGC28761 antibody

  see all

Anti-ATL3 antibody images

• 

  Western blot - Anti-ATL3 antibody (ab117834)
  All lanes : Anti-ATL3 antibody (ab117834) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : Jurkat whole cell lysate at 50 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  Lane 5 : NIH3T3 whole cell lysate at 50 µg
  
  developed using the ECL technique
  
  Predicted band size : 61 kDa
  
  
  Exposure time : 30 seconds
• 

  Immunoprecipitation - Anti-ATL3 antibody (ab117834)
  Detection of Human ATL3 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab117834 at 6 µg/mg of lysate. Subsequent WB detection was done using 117834 at 1 µg/ml. ECL detection. Exposure time: 10 seconds.