Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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ab78 has been referenced in 13 publications.
Publishing research using ab78? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-ATM antibody [2C1 (1A1)] (ab78) at 1/2000 dilution
Lane 1 : 50ug lymphoblastoid nuclear lysate, anti-beta actin antibody.
Lane 2 : 25ug lymphoblastoid nuclear lysate, anti-beta actin antibody.
Lane 3 : No ATM negative control, anti-beta actin antibody.
Secondary
Anti-mouse secondary antibody at 1/2000 dilution
Performed under reducing conditions.
Predicted band size : 350 kDa
Arrows denote the location of the 45kDa beta actin protein, and the 350kDa ATM protein.
Simona Cavalieri, University of Turin, Italy
Predicted band size : 350 kDa
Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (ab78) by western blot.
Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (ab78) by immunoprecipitation.
Predicted band size : 350 kDa
Detection of ATM (Anti-ATM 2C1, ab78) or ATR (Anti-ATR 2B5,ab4471) by western blot in human Raji whole cell extract.
ab78 (2µg/ml) staining ATM in human colonic mucosa, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of mucosal epithelium and lymphocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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