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Western blot - ATM antibody [2C1 (1A1)] (ab78)
![Western blot - ATM antibody [2C1 (1A1)] (ab78)](/ps/datasheet/images/ab78_1.jpg)
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Western blot - ATM antibody [2C1 (1A1)] (ab78)
![Western blot - ATM antibody [2C1 (1A1)] (ab78)](/ps/datasheet/images/ab78_2.jpg)
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Immunoprecipitation - ATM antibody [2C1 (1A1)] (ab78)
![Immunoprecipitation - ATM antibody [2C1 (1A1)] (ab78)](/ps/datasheet/images/ab78_3.jpg)
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Product Name
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ATM antibody [2C1 (1A1)]
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See all ATM antibodies (18)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2C1 (1A1)] to ATM
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Immunogen
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Fusion protein expressed in E. coli corresponding to amino acids 2577-3056.
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Reacts with
(species key)
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Hu, Ms, Rat, Mk
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 1 - 10 µg/ml.
WB: 1/2000. Detects a band of approximately 350 kDa (predicted molecular weight: 350 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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lymphoblastoid nuclear lysate
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Cellular localization
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Nuclear. Found also in endocytic vesicles in association with beta adaptin.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Relevance
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ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
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ATM antibody [2C1 (1A1)] (ab78) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2C1 (1A1)
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Myeloma
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NS1
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Isotype
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IgG1
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Light chain type
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kappa
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Storage buffer
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Preservative: None
Constituents: 10mM PBS, pH 7.4
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Purification notes
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Purified from ascities fluid by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATM antibody [2C1 (1A1)] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATM antibody [2C1 (1A1)] images, references, products related to ab78 and other tools.
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ATM antibody [2C1 (1A1)] images:
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 Western blot - ATM antibody [2C1 (1A1)] (ab78)
All lanes : ATM antibody [2C1 (1A1)] (ab78) at 1/2000 dilution
Lane 1 : 50ug lymphoblastoid nuclear lysate, anti-beta actin antibody.
Lane 2 : 25ug lymphoblastoid nuclear lysate, anti-beta actin antibody.
Lane 3 : No ATM negative control, anti-beta actin antibody.
Secondary
Anti-mouse secondary antibody at 1/2000 dilution
Performed under reducing conditions.
Predicted band size : 350 kDa
Arrows denote the location of the 45kDa beta actin protein, and the 350kDa ATM protein. Simona Cavalieri, University of Turin, Italy
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Western blot - ATM antibody [2C1 (1A1)] (ab78)
Predicted band size : 350 kDa
Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (ab78) by western blot.
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Immunoprecipitation - ATM antibody [2C1 (1A1)] (ab78)
Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (ab78) by immunoprecipitation.
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Western blot - ATM antibody [2C1 (1A1)] (ab78)
![Western blot - ATM antibody [2C1 (1A1)] (ab78)](/ps/datasheet/images/ab78_4.jpg)
Predicted band size : 350 kDa
Detection of ATM (Anti-ATM 2C1, ab78) or ATR (Anti-ATR 2B5,ab4471) by western blot in human Raji whole cell extract.
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References for ATM antibody [2C1 (1A1)] (ab78)
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This product has been used in: (two most recent references)
Mattiussi S et al. Homeodomain interacting protein kinase 2 activation compromises endothelial cell response to laminar flow: protective role of p21(waf1,cip1,sdi1). PLoS One 4:e6603 (2009). WB; Human. PubMed: 19668373
Winter M et al. Control of HIPK2 stability by ubiquitin ligase Siah-1 and checkpoint kinases ATM and ATR. Nat Cell Biol 10:812-24 (2008). WB; Human. PubMed: 18536714
See all 9 publication references for this product.
If you publish research using ab78 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ATM
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ATM antibody [2C1 (1A1)] - more information
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Customer reviews (feedback) regarding ATM antibody [2C1 (1A1)] |
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Price and availability of products related to ATM antibody [2C1 (1A1)] |
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ATM antibody [2C1 (1A1)] - related products:
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ATM antibody [2C1 (1A1)] - other tools:
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Contact Abcam with a Technical Enquiry about ATM antibody [2C1 (1A1)] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - ATM antibody [2C1 (1A1)] (ab78)](/index.html?datasheet=78#ab78_1.jpg)
![Western blot - ATM antibody [2C1 (1A1)] (ab78)](/index.html?datasheet=78#ab78_2.jpg)
![Immunoprecipitation - ATM antibody [2C1 (1A1)] (ab78)](/index.html?datasheet=78#ab78_3.jpg)
