|
|
|
|
|
Product Name
|
ATM antibody [5C2]
|
| |
See all ATM antibodies (18)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [5C2] to ATM
|
|
Immunogen
|
Recombinant fragment, corresponding to amino acids 980-1512 of Human ATM
|
|
Reacts with
(species key)
|
Hu, Ms, Rat, Mk
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 350 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Raji or Akata whole cell lysate.
|
|
Cellular localization
|
Nuclear. Found also in endocytic vesicles in association with beta adaptin.
|
|
Research areas
|
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
|
|
Relevance
|
ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
5C2
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Storage buffer
|
Preservative: None
Constituents: 10mM PBS, pH 7.4
|
|
Purification notes
|
Purified from ascities fluid by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATM antibody [5C2] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
References for ATM antibody [5C2] (ab2618)
|
|
This product has been used in: (two most recent references)
Rubinek T et al. Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells. Am J Physiol Cell Physiol 293:C1082-92 (2007). PubMed: 17626243
Adams KE et al. Recruitment of ATR to sites of ionising radiation-induced DNA damage requires ATM and components of the MRN protein complex. Oncogene 25:3894-904 (2006). PubMed: 16474843
See all 4 publication references for this product.
If you publish research using ab2618 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to ATM
|
| |
ATM antibody [5C2] - more information
|
Customer reviews (feedback) regarding ATM antibody [5C2] |
Customer FAQs regarding ATM antibody [5C2] |
Protocols for ATM antibody [5C2] |
Price and availability of products related to ATM antibody [5C2] |
| |
ATM antibody [5C2] - related products:
|
|
|
| |
ATM antibody [5C2] - other tools:
|
Contact Abcam with a Technical Enquiry about ATM antibody [5C2] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
