Anti-ATM antibody [ATM 11G12] (ab31842)

Overview

• Product nameAnti-ATM antibody [ATM 11G12]See all ATM primary antibodies ...
• Description
  Mouse monoclonal [ATM 11G12] to ATM
• Tested applicationsICC/IF, Flow Cyt, WB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 992-1144 of ATM

• Positive controlLymphoblastoid cell line lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberATM 11G12
• MyelomaSp2/0-Ag14
• IsotypeIgG1
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • ATM / ATR

Applications

Target

• FunctionSerine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
• Tissue specificityFound in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
• Involvement in diseaseDefects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
  Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
  Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
  Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
• Sequence similaritiesBelongs to the PI3/PI4-kinase family. ATM subfamily.
  Contains 1 FAT domain.
  Contains 1 FATC domain.
  Contains 1 PI3K/PI4K domain.
• DomainThe FATC domain is required for interaction with KAT5.
• Post-translational
  modificationsPhosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
  Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
• Cellular localizationNucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.

Target information above from: UniProt accession Q13315 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 472 Human
  • Entrez Gene: 472 Human
  • Omim: 607585 Human
  • SwissProt: Q13315 Human
  • SwissProt: Q13315 Human
  • Unigene: 367437 Human

• Alternative names
  A-T mutated antibodyA-T mutated homolog antibodyAT complementation group A antibody

  AT complementation group C antibodyAT complementation group D antibodyAT complementation group E antibodyAT mutated antibodyAT protein antibodyAT1 antibodyATA antibodyAtaxia telangiectasia gene mutated in human beings antibodyAtaxia telangiectasia mutated antibodyAtaxia telangiectasia mutated gene antibodyAtaxia telangiectasia mutated homolog (human) antibodyAtaxia telangiectasia mutated homolog antibodyATC antibodyATD antibodyATDC antibodyATE antibodyATM antibodyATM_HUMAN antibodyDKFZp781A0353 antibodyHuman phosphatidylinositol 3 kinase homolog antibodyMGC74674 antibodyOTTHUMP00000232981 antibodySerine protein kinase ATM antibodySerine-protein kinase ATM antibodySerine/threonine-protein kinase ATM antibodyT cell prolymphocytic leukemia antibodyTefu antibodyTEL1 antibodyTEL1, telomere maintenance 1, homolog antibodyTELO1 antibodyTelomere fusion protein antibodyTPLL antibody

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Anti-ATM antibody [ATM 11G12] images

• 

  Western blot - ATM antibody [ATM 11G12] (ab31842)
  All lanes : Anti-ATM antibody [ATM 11G12] (ab31842) at 1/500 dilution
  
  Lane 1 : Lysate of lymphoblastoid cell line from a normal patient showing ATM
  Lane 2 : Lysate from a classical A-T patient with two truncating ATM mutations showing no ATM protein expression
  Lane 3 : Lysate from an A-T patient carrying a splicing mutation that results in expression of ~5% of normal ATM
  Lane 4 : Lysate of lymphoblastoid cell line from a normal patient showing ATM
  
  
  Predicted band size : 350 kDa
  
• 

  Flow Cytometry - Anti-ATM antibody [ATM 11G12] (ab31842)
  Overlay histogram showing HeLa cells stained with ab31842 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31842, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.