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Western blot - ATM (phospho S15) antibody [MAT3-4G10/8] (ab59541)
![Western blot - ATM (phospho S15) antibody [MAT3-4G10/8] (ab59541)](/ps/datasheet/images/ab59541.jpg)
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Product Name
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ATM antibody [MAT3-4G10/8]
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See all ATM antibodies (18)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MAT3-4G10/8] to ATM
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Immunogen
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Synthetic peptide: YSDKKSTDEQEKRSPTFEEGSQ conjugated to KLH by a Cysteine residue linker at N-terminus, corresponding to amino acids 1967-1988 of Mouse ATM.
BLAST 'YSDKKSTDEQEKRSPTFEEGSQ' with  or 
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody recognizes ATM.
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Tested applications
(see key)
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I-ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at an assay dependent dilution.
I-ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 350 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Total extract of HEK293T cells.
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Cellular localization
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Nuclear. Found also in endocytic vesicles in association with beta adaptin.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Relevance
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ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
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ATM antibody [MAT3-4G10/8] (ab59541) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MAT3-4G10/8
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Myeloma
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NS0
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Isotype
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IgG1
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified immunoglobulin.
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATM antibody [MAT3-4G10/8] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATM antibody [MAT3-4G10/8] images, references, products related to ab59541 and other tools.
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ATM antibody [MAT3-4G10/8] images:
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 Western blot - ATM (phospho S15) antibody [MAT3-4G10/8] (ab59541)
Lane 1 : ATM antibody [MAT3-4G10/8] (ab59541) at 0.5 µg/ml
Lane 2 : ATM antibody [MAT3-4G10/8] (ab59541) at 0.25 µg/ml
Lane 3 : ATM antibody [MAT3-4G10/8] (ab59541) at 0.125 µg/ml
Lane 1 : Total cell extract of HEK-293T cells
Lane 2 : Total cell extract of HEK-293T cells
Lane 3 : Total cell extract of HEK-293T cells
Secondary
Goat Anti-Mouse, Peroxidase conjugate
Predicted band size : 350 kDa
Observed band size : 300 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to ATM
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ATM antibody [MAT3-4G10/8] - more information
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Customer reviews (feedback) regarding ATM antibody [MAT3-4G10/8] |
Customer FAQs regarding ATM antibody [MAT3-4G10/8] |
Protocols for ATM antibody [MAT3-4G10/8] |
Price and availability of products related to ATM antibody [MAT3-4G10/8] |
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ATM antibody [MAT3-4G10/8] - related products:
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ATM antibody [MAT3-4G10/8] - other tools:
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Contact Abcam with a Technical Enquiry about ATM antibody [MAT3-4G10/8] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - ATM (phospho S15) antibody [MAT3-4G10/8] (ab59541)](/index.html?datasheet=59541#ab59541.jpg)
