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Western blot - ATM antibody (ab2631)
(enlarge)
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Product Name
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ATM antibody
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See all ATM antibodies (18)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ATM
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Immunogen
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Synthetic peptide (Human) - which represents a portion of human Ataxia Telangiectasia Mutated (ATM) proteinencoded within exon 53.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 10 - 20 µg/ml.
WB: 1/2500 - 1/10000. Detects a band of approximately 350 kDa (predicted molecular weight: 350 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Positive controls: Human L-40 or mouse MF-10 wildtype whole cell lysates. Negative controls: Human L-6 or mouse MF-12 ATM deficient whole cell lysates.
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Cellular localization
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Nuclear. Found also in endocytic vesicles in association with beta adaptin.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Relevance
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ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Affinity purified using the immunising peptide immobilized on solid support.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATM antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATM antibody images, references, products related to ab2631 and other tools.
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ATM antibody images:
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 Western blot - ATM antibody (ab2631)
Predicted band size : 350 kDa
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References for ATM antibody (ab2631)
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This product has been used in: (two most recent references)
Stephan H et al. Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. Nucleic Acids Res 37:6028-41 (2009). WB; Human. PubMed: 19671522
Turner NC et al. A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor. EMBO J : (2008). WB; Human. PubMed: 18388863
See all 3 publication references for this product.
If you publish research using ab2631 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ATM
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ATM antibody - more information
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Customer reviews (feedback) regarding ATM antibody |
Customer FAQs regarding ATM antibody |
Protocols for ATM antibody |
Price and availability of products related to ATM antibody |
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ATM antibody - related products:
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ATM antibody - other tools:
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Contact Abcam with a Technical Enquiry about ATM antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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