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Western blot - ATM antibody (ab91)
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- ATM antibody (ab91)
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Western blot - ATM antibody (ab91)
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Product Name
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ATM antibody
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See all ATM antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATM
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Immunogen
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Recombinant fragment expressed in E. coli, corresponding to amino acids 2138-2739 (the RAD3 domain) of Human ATM.
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Reacts with
(species key)
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Hu, Rat
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Specificity
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This antibody is specific for ATM.
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Tested applications
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/750.
IHC-P: 1/750.
IP: 1/333.
WB: 1/2500. Detects a band of approximately 350 kDa (predicted molecular weight: 350 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear. Found also in endocytic vesicles in association with beta adaptin.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Relevance
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ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Phosphate buffered saline
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATM antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATM antibody images, references, products related to ab91 and other tools.
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ATM antibody images:
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 Western blot - ATM antibody (ab91)
All lanes : ATM antibody (ab91) at 1/2500 dilution
Lane 1 : 25ug lymphoblastoid nuclear lysate
Lane 2 : 25ug lymphoblastoid nuclear lysate from an AT patient (10% amount of ATM protein as normal individual).
Secondary
Anti-rabbit secondary antibody at 1/2000 dilution
Performed under reducing conditions.
Predicted band size : 350 kDa
Observed band size : 350 kDa
Simona Cavalieri, University of Turin, Italy
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- ATM antibody (ab91)
Immunohistochemical staining using ab91. Antibody dilution was 1:500.
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Western blot - ATM antibody (ab91)
Predicted band size : 350 kDa
Western blot using ab91. Lane 1: A-T patient sample
Lane 2: Non A-T patient sample Antibody dilution is 1:1000.
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Western blot - ATM antibody (ab91)
Predicted band size : 350 kDa
Detection of ATM in HeLa nuclear extracts using ab91. Antibody concentration: 1 ug/ml. Detection: 2 minute exposure.
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References for ATM antibody (ab91)
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This product has been used in: (two most recent references)
Zheng L et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 37:2645-57 (2009). WB; Human. PubMed: 19270065
Vijayakumar S et al. Phosphorylation of human DNA ligase I regulates its interaction with replication factor C and its participation in DNA replication and DNA repair. Mol Cell Biol 29:2042-52 (2009). WB; Human. PubMed: 19223468
See all 5 publication references for this product.
If you publish research using ab91 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ATM
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ATM antibody - more information
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Customer reviews (feedback) regarding ATM antibody |
Customer FAQs regarding ATM antibody |
Protocols for ATM antibody |
Price and availability of products related to ATM antibody |
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ATM antibody - related products:
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ATM antibody - other tools:
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Contact Abcam with a Technical Enquiry about ATM antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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