Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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ab36810 has been referenced in 5 publications.
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All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 10 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Extract from Patient with Ataxia-Telangiectasia Whole Cell Lysate
Lane 3 : Irradiated HeLa Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 370 kDa
Observed band size : 370 kDa
Additional bands at : 100 kDa,110 kDa,145 kDa,200 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 1/1000 dilution
Lane 1 : Control lane
Lane 2 : Irradiated Human fibroblasts (10 Gy gamma-irradiation)
Lane 3 : Molecular weight marker
Lane 4 : Peroxidated Human fibroblasts (300 µM hydrogen peroxide)
Lane 5 : Peroxidated Human fibroblasts (1 mM hydrogen peroxide)
Lane 6 : Peroxidated Human fibroblasts (10 mM hydrogen peroxide)
developed using the ECL technique
Predicted band size : 370 kDa
Observed band size : 370 kDa
Lysate loading concentration: 40µg
Ab36810staining Human colon. Staining is localised to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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