Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810)

Overview

• Product nameAnti-ATM (phospho S1981) antibody [10H11.E12]See all ATM primary antibodies ...
• Description
  Mouse monoclonal [10H11.E12] to ATM (phospho S1981)
• Specificityab36810 is specific for the human ATM kinase.
• Tested applicationsFlow Cyt, WB, ICC, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide (Human) with a phosphorylated serine (1981)

• Positive controlIrradiated normal Human fibroblasts (no reactivity against non-irradiated cell extracts).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number10H11.E12
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • ATM / ATR

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• FunctionSerine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
• Tissue specificityFound in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
• Involvement in diseaseDefects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
  Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
  Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
  Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
• Sequence similaritiesBelongs to the PI3/PI4-kinase family. ATM subfamily.
  Contains 1 FAT domain.
  Contains 1 FATC domain.
  Contains 1 PI3K/PI4K domain.
• DomainThe FATC domain is required for interaction with KAT5.
• Post-translational
  modificationsPhosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
  Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
• Cellular localizationNucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.

Target information above from: UniProt accession Q13315 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 472 Human
  • Entrez Gene: 472 Human
  • Entrez Gene: 11920 Mouse
  • Entrez Gene: 300711 Rat
  • Omim: 607585 Human
  • SwissProt: Q13315 Human
  • SwissProt: Q13315 Human
  • SwissProt: Q62388 Mouse

  • SwissProt: P17764 Rat
  • Unigene: 367437 Human
  • Unigene: 5088 Mouse
  • Unigene: 214048 Rat

  see all

• Alternative names
  A-T mutated antibodyA-T mutated homolog antibodyAT complementation group A antibody

  AT complementation group C antibodyAT complementation group D antibodyAT complementation group E antibodyAT mutated antibodyAT protein antibodyAT1 antibodyATA antibodyAtaxia telangiectasia gene mutated in human beings antibodyAtaxia telangiectasia mutated antibodyAtaxia telangiectasia mutated gene antibodyAtaxia telangiectasia mutated homolog (human) antibodyAtaxia telangiectasia mutated homolog antibodyATC antibodyATD antibodyATDC antibodyATE antibodyATM antibodyATM_HUMAN antibodyDKFZp781A0353 antibodyHuman phosphatidylinositol 3 kinase homolog antibodyMGC74674 antibodyOTTHUMP00000232981 antibodySerine protein kinase ATM antibodySerine-protein kinase ATM antibodySerine/threonine-protein kinase ATM antibodyT cell prolymphocytic leukemia antibodyTefu antibodyTEL1 antibodyTEL1, telomere maintenance 1, homolog antibodyTELO1 antibodyTelomere fusion protein antibodyTPLL antibody

  see all

Anti-ATM (phospho S1981) antibody [10H11.E12] images

• 

  Western blot - ATM (phospho S1981) antibody [10H11.E12] (ab36810)
  All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 10 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Extract from Patient with Ataxia-Telangiectasia Whole Cell Lysate
  Lane 3 : Irradiated HeLa Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under non-reducing conditions.
  
  Predicted band size : 370 kDa
  Observed band size : 370 kDa
  Additional bands at : 100 kDa,110 kDa,145 kDa,200 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes
• 

  Western blot - ATM Kinase antibody [10H11.E12] (ab36810)
  All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 1/1000 dilution
  
  Lane 1 : Control lane
  Lane 2 : Irradiated Human fibroblasts (10 Gy gamma-irradiation)
  Lane 3 : Molecular weight marker
  Lane 4 : Peroxidated Human fibroblasts (300 µM hydrogen peroxide)
  Lane 5 : Peroxidated Human fibroblasts (1 mM hydrogen peroxide)
  Lane 6 : Peroxidated Human fibroblasts (10 mM hydrogen peroxide)
  
  developed using the ECL technique
  
  Predicted band size : 370 kDa
  Observed band size : 370 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATM (phospho S1981 + S1981) antibody [10H11.E12] (ab36810)
  Ab36810staining Human colon. Staining is localised to the nucleus.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Flow Cytometry-Anti-ATM (phospho S1981) antibody [10H11.E12](ab36810)
  Overlay histogram showing HeLa cells stained with ab36810 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36810, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.