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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)
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Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab81292 for help.
There are no answered questions for ab81292
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATM (phospho S1981) antibody [EP1890Y] (ab81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATM (phospho S1981) antibody [EP1890Y] (ab81292)
ab81292, 1/100, staining Human gastric carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
![Western blot - ATM (phospho S1981) antibody [EP1890Y] (ab81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-5.jpg)
Western blot - ATM (phospho S1981) antibody [EP1890Y] (ab81292)
All lanes : Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) at 1/10000 dilution
Lane 1 : HEK293 cell lysates untreated.
Lane 2 : HEK293 cell lysates treated with doxorubicin.
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : HRP labelled goat anti-rabbit at 1/2000 dilution
Lane 2 : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 351 kDa
Observed band size : 370 kDa (why is the actual band size different from the predicted?)
![Immunocytochemistry/ Immunofluorescence - ATM (phospho S1981) antibody [EP1890Y] (ab81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-9.jpg)
Immunocytochemistry/ Immunofluorescence - ATM (phospho S1981) antibody [EP1890Y] (ab81292)
ICC/IF image of ab81292 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81292,1µg/ml) overnight at +4ºC. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![ATM (phospho S1981) antibody [EP1890Y] for WB in Human (81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-6.jpg)
ATM (phospho S1981) antibody [EP1890Y] for WB in Human (81292)
![ATM (phospho S1981) antibody [EP1890Y] for Western blot in Human (81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-8.jpg)
ATM (phospho S1981) antibody [EP1890Y] for Western blot in Human (81292)
![Anti-ATM (phospho S1981) antibody [EP1890Y] for Immunocytochemistry/ Immunofluorescence in Human (81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-26.JPG)
Anti-ATM (phospho S1981) antibody [EP1890Y] for Immunocytochemistry/ Immunofluorescence in Human (81292)
![Anti-ATM (phospho S1981) antibody [EP1890Y] for Immunocytochemistry/ Immunofluorescence in Human (81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-10.jpg)
Anti-ATM (phospho S1981) antibody [EP1890Y] for Immunocytochemistry/ Immunofluorescence in Human (81292)
![Anti-ATM (phospho S1981) antibody [EP1890Y] for Western blot in Human (81292)](/ps/datasheet/images/81/ab81292/ATM-Primary-antibodies-ab81292-19.jpg)
Anti-ATM (phospho S1981) antibody [EP1890Y] for Western blot in Human (81292)
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