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Does not react withMouse, Rat
A synthetic peptide corresponding to residues surrounding serine 1981 of human ATM was used as an immunogen.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A 40 µl trial size is available to purchase for this antibody.
Our Abpromise guarantee covers the use of ab81292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 370 kDa (predicted molecular weight: 351 kDa).|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
ab81292 staining ATM (phospho S1981) in Human primary fibroblasts treated with IR (5Gy) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 2 hours at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 14 hours at 4°C. A goat anti rabbit Alexa Fluor® 488 (IgG polyclonal; ab150077 (1/1000) was used as the secondary antibody.
Left - ATM (phospho S1981), Right - DAPI + merge.
ab81292 staining ATM in Human HeLa cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in TBS-Tween20 + 5% BSA) for 1 hour at 22°C. A TRITC-conjugated Donkey anti-rabbit IgG polyclonal (1/150) was used as the secondary antibody. Cells treated with 10 Gy IR and allowed to recover for 30 minutes before fixed.
Image courtesy Anonymous Abreview
ICC/IF image of ab81292 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81292,1µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (IgG - H&L, pre-adsorbed: (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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