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Does not react withMouse, Rat
A synthetic peptide corresponding to residues surrounding serine 1981 of human ATM was used as an immunogen.
Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.
Our Abpromise guarantee covers the use of ab81292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 370 kDa (predicted molecular weight: 351 kDa).|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
ab81292 staining ATM (phospho S1981) in Human primary fibroblasts treated with IR (5Gy) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 2 hours at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Left - ATM (phospho S1981), Right - DAPI + merge.
ab81292 staining ATM in Human HeLa cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in TBS-Tween20 + 5% BSA) for 1 hour at 22°C. A TRITC-conjugated Donkey anti-rabbit IgG polyclonal (1/150) was used as the secondary antibody. Cells treated with 10 Gy IR and allowed to recover for 30 minutes before fixed.
Image courtesy Anonymous Abreview
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