Anti-ATM (phospho S794) antibody (ab119799)
Key features and details
- Rabbit polyclonal to ATM (phospho S794)
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATM (phospho S794) antibody
See all ATM primary antibodies -
Description
Rabbit polyclonal to ATM (phospho S794) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human ATM (phospho S794).
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Positive control
- Jurkat, A431, HeLa, and Rat PC12 cells, treated with Calyculin A.
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General notes
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 351 kDa.
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ICC |
1/200.
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Notes |
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WB
1/1000. Predicted molecular weight: 351 kDa. |
ICC
1/200. |
Target
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Function
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. -
Tissue specificity
Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes. -
Involvement in disease
Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure. -
Sequence similarities
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 1 PI3K/PI4K domain. -
Domain
The FATC domain is required for interaction with KAT5. -
Post-translational
modificationsPhosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. -
Cellular localization
Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin. - Information by UniProt
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Database links
- Entrez Gene: 472 Human
- Entrez Gene: 11920 Mouse
- Omim: 607585 Human
- SwissProt: Q13315 Human
- SwissProt: Q62388 Mouse
- Unigene: 367437 Human
- Unigene: 5088 Mouse
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Alternative names
- A-T mutated antibody
- A-T mutated homolog antibody
- AT mutated antibody
see all
Images
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ab119799 at 1/200 dilution staining ATM (phospho S794) in A431 cells treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to DyLight 594.
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Lanes 1-2 : Anti-ATM (phospho S794) antibody (ab119799) at 1/1000 dilution
Lanes 3-4 : Anti-
ATM (C-Terminal) at 1/1000 dilution
Lanes 5-6 : Anti-ATM (phospho S1981) at 1/1000 dilution
Lanes 1 & 3 & 5 : Human A431 cells treated with Calyculin A
(100 nM) for 30 min
Lanes 2 & 4 & 6 : Human A431 cells treated with Calyculin A
(100 nM) for 30 min, then treated with lambda phosphatase
Predicted band size: 351 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab119799 has been referenced in 1 publication.
- Zhang W et al. Nucleolar stress induces a senescence-like phenotype in smooth muscle cells and promotes development of vascular degeneration. Aging (Albany NY) 12:22174-22198 (2020). PubMed: 33146634