Overview

  • Product nameATP Assay Kit (Colorimetric/Fluorometric)
    See all ATP kits
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay typeQuantitative
  • Sensitivity
    < 1 µM
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Product overview

    Abcam's ATP Assay Kit (Colorimetric/Fluorometric) is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (?max = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria.

  • Tested applicationsFunctional Studiesmore details

Properties

  • Alternative names
    • Adenosine 5' triphosphate

Applications

Our Abpromise guarantee covers the use of ab83355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

ATP Assay Kit (Colorimetric/Fluorometric) images

  • Example of fluorometric ATP assay standard curve.
  • Example of colorimetric ATP assay standard curve.
  • Quantitation of ATP in fish liver (2.5µl of 10 times diluted sample), fish muscle (5µl of 10 times diluted sample) and Jurkat cell lysate (5 ul) using fluorometric assay. Samples were spiked with known amounts of ATP (300pmol).

Protocols

References for ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)

This product has been referenced in:
  • Goncharov DA  et al. Mammalian target of rapamycin complex 2 (mTORC2) coordinates pulmonary artery smooth muscle cell metabolism, proliferation, and survival in pulmonary arterial hypertension. Circulation 129:864-74 (2014). Functional Studies . Read more (PubMed: 24270265) »
  • Jitschin R  et al. Mitochondrial metabolism contributes to oxidative stress and reveals therapeutic targets in chronic lymphocytic leukemia. Blood 123:2663-72 (2014). Functional Studies . Read more (PubMed: 24553174) »

See all 16 Publications for this product

Product Wall

Unfortunately, we have not tested the interference of desferrioxamine with this kit.

Thank you for contacting us at Abcam Scientific Support.

I am happy to assist with your protocol inquiries.

1) The instruction booklet says to homogenize of the sample with ATP assay buffer first and then deproteinize using PCA and ...

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Since there are enzymes in the assay which might need metal ions for function, we do not recommend metal chelators like EDTA for blood collection. Heparin is a better choice.


Generally, fresh samples are best, but frozen samples can be used with this kit. We suggest snap-freezing cells or tissue, and deproteinizing lysates before freezing, for best results.

Using a 561 nm excitation rather than the recommended 535 nm would compromise the results. Typically, bandpass filters are +/- 10nm, so you're 561 nm excitation would have a flexibility of 551 - 571 nm. If you do not have the means of exciting at 535 n...

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For the colorimetric assay, use a clear plate. For the fluorometric assay a clear or a white plate can be used. Use flat bottom plates.

This kit will detects total ATP.

Yes, you will have to use a homogenizer to ensure proper tissue lysis in the assay buffer. Any type of homogenizer that you prefer can be used, and as shown on the datasheet, we recommend deproteinizing sample isolation preferably with PCA rather than ...

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Yes, your 562-595 nm filters should work fine for this kit.

This kit has been tested using both fluorometric and colourimetric methods and can detect as low as 1 µM of ATP in various samples. Therefore, it is highly unlikely that this kit will be able to detect ATP in your samples as 1 nM is outside of it...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"