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ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (ODmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.
This kit can detect as low as 1 µM of ATP in various samples.
If you require a more sensitive product, we recommend Luminescent ATP Detection Assay Kit (ab113849), which can detect as low as 1 pM of ATP.
Visit our FAQs page for tips and troubleshooting.
ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria.
|ATP Assay Buffer||WM||1 x 25ml|
|ATP Converter (lyophilized)||Blue||1 vial|
|ATP Probe (in DMSO)||Red||1 x 200µl|
|ATP Standard (1 µmol; lyophilized)||Yellow||1 vial|
|Developer Mix (lyophilized)||Green||1 vial|
Our Abpromise guarantee covers the use of ab83355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Quantitation of ATP in fish liver (2.5µl of 10 times diluted sample), fish muscle (5µl of 10 times diluted sample) and Jurkat cell lysate (5 ul) using fluorometric assay. Samples were spiked with known amounts of ATP (300pmol).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"