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Product name
ATP Synthase Immunocapture Kit
See all ATP Synthase products (3) ...
Assay type
Quantitative
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria.
ab109715 allows isolation of the ATP synthase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for ab109715 include but are not limited to examining alterations of ATP synthase subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Tested applications
Properties
Storage instructions
Store at +4°C.
| Components | 250 µg | 500 µg | 750 µg |
|---|---|---|---|
| monoclonal ATP synthase antibody irreversibly crosslinked to protein G-agarose beads | 1 x 250µg | 1 x 500µg | 1 x 750µg |
Research areas
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
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Western blot - ATP Synthase Immunocapture Kit (ab109715)
(enlarge)
Alternative names
- MS541
- MS543
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
IPIP
ATP Synthase Immunocapture Kit images:
Western blot - ATP Synthase Immunocapture Kit (ab109715)
Using the immunoprecipitation protocol provided, ATP synthase was isolated from various samples by antibody ab109867 crosslinked to protein G-agarose beads (ab109715). Protein bands identities were confirmed by mass spectrometry.
Protocols
References for ATP Synthase Immunocapture Kit (ab109715)
This product has been referenced in:
- Bao J et al. SIRT3 is regulated by nutrient excess and modulates hepatic susceptibility to lipotoxicity. Free Radic Biol Med 49:1230-7 (2010).Read more (PubMed: 20647045) »
- Giorgio V et al. The ectopic F(O)F(1) ATP synthase of rat liver is modulated in acute cholestasis by the inhibitor protein IF1. J Bioenerg Biomembr 42:117-23 (2010).Read more (PubMed: 20180002) »
See all 4 publications for this product
Publishing research using ab109715? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"