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Anti-ATP Synthase beta antibody (ab43176)

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Overview

  • Product nameAnti-ATP Synthase beta antibody
  • Description
    Chicken polyclonal to ATP Synthase beta
  • SpecificityThis antibody will detect the mitochondrial form of the F1 ATP Synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. This antibody will not cross-react with -V-type ATP Synthase, found mainly in Archaea.
  • Tested applicationsWB more details
  • Species reactivity
    Chloroplasts, mitochondria and most bacteria.
  • Immunogen

    Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the ATP Synthase beta protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab43176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/5000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).

    Immunolocalization: Use at an assay-dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceMitochondrial membrane ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk.
    • Cellular localizationMitochondrion. Mitochondrion inner membrane. Note=Peripheral membrane protein.
    • Alternative names
        ATP 5B antibodyATP synthase beta chain 1, mitochondrial antibodyATP synthase beta chain 2, mitochondrial antibody
        ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibodyATP synthase subunit beta mitochondrial antibodyATP5B antibodyATPMB antibodyATPSB antibodyMGC5231 antibodyMitochondrial ATP synthetase beta subunit antibody
      see all

    Anti-ATP Synthase beta antibody images

    • Western blot - AtpB antibody (ab43176)
      Western blot - AtpB antibody (ab43176)
      All lanes : Anti-ATP Synthase beta antibody (ab43176) at 1/5000 dilution

      Lane 1 : Markers 20, 30, 40, 50, 60, 80, 100, 120, 220kDa.
      Lane 2 : Extract of Synechocystis
      Lane 3 : Isolated thylakoid fraction of Spinach

      Secondary
      HRP-conjugated anti IgY, from Abcam at 1/50000 dilution
      developed using the ECL technique

      Predicted band size : 59 kDa
      Observed band size : 52,54 kDa (why is the actual band size different from the predicted?)

    References for Anti-ATP Synthase beta antibody (ab43176)

    ab43176 has not yet been referenced specifically in any publications.

    Publishing research using ab43176 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab43176 Submit a Question for ab43176

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    Dear Madam/Sir,   I have recently read an interesting article regarding the regulatory role of PKCzeta isoform on F0F1 ATP synthase beta subunit. It claimed that PKCzeta is able to phosphorilate the beta subunit of the F0F1 ATP synthase and hence, regu...

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    Thank you for your inquiry. I have conducted a search for you and found the following antibodies: ab43176 - Rabbit polyclonal to PKC zeta ab4137 - Chicken polyclonal to ATP Synthase beta ab51157 - Rabbit polyclonal to PKC zeta ab59412 - Rabbit ...

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    Hi there, Following my failure with ab5432 while trying it for the locust protein, I'm going to test another antibody. I'm wondering if you have the sequence of the peptide used to produce ab43177 and ab43176. unfortunately I don't have the sequence of...

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    Thank you once again for your message and for your patience in waiting for a response. I have now received further information from the originator of these ATP Synthase beta antibodies (ab43177 and ab43176). Although they are not able to provide the im...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"