Anti-ATP citrate lyase antibody [3G8] (ab118150)

Overview

• Product nameAnti-ATP citrate lyase antibody [3G8]See all ATP citrate lyase primary antibodies ...
• Description
  Mouse monoclonal [3G8] to ATP citrate lyase
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length Human ATP citrate lyase produced in HEK293T cells (NP_001087).

• Positive controlHEK293T cell lysate transfected with pCMV6-ENTRY ATP citrate lyase transfected; Human breast adenocarcinoma, colon, colon adenocarcinoma, kidney, liver, liver carcinoma, lung, ovary adenocarcinoma, pancreas, pancreas carcinoma, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate, prostate carcinoma, and bladder tissies; COS7 cells transiently transfected by pCMV6-ENTRY ATP citrate lyase.
• General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 1% BSA, 50% Glycerol
• Concentration information loading...
• PurityProtein G purified
• Purification notesab118150 is purified from Mouse ascites fluid by affinity chromatography.
• Clonality Monoclonal
• Clone number3G8
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

Applications

Target

• FunctionATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
• Sequence similaritiesIn the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
  In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P53396 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 47 Human
  • Omim: 108728 Human
  • SwissProt: P53396 Human
  • Unigene: 387567 Human

• Alternative names
  ACL antibodyAcly antibodyACLY_HUMAN antibody

  ATP citrate (pro-S) lyase antibodyATP citrate lyase antibodyATP citrate synthase antibodyATP-citrate (pro-S-)-lyase antibodyATP-citrate synthase antibodyATPcitrate synthase antibodyATPCL antibodyCitrate cleavage enzyme antibodyCLATP antibodyOTTHUMP00000164773 antibody

  see all

Anti-ATP citrate lyase antibody [3G8] images

• 

  Western blot - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  All lanes : Anti-ATP citrate lyase antibody [3G8] (ab118150) at 1/2000 dilution
  
  Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ATP citrate lyase cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 120 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human colon tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human liver tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human liver carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human lung tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry..
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human thyroid tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human prostate tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry..
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  ab118150 at 1/50 dilution staining ATP citrate lyase in Paraffin-embedded Human bladder tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [3G8] (ab118150)
  Immunofluorescent staining of COS7 cells transiently transfected by ATP citrate lyase using ab118150 at a dilution of 1/100.