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All lanes : Anti-ATP citrate lyase antibody [EP737Y] (ab53007) at 1/1000 dilution
Lane 1 : (A) 3T3 cell lysate (untreated cells)
Lane 2 : (B) 3T3 cell lysate (insulin treated cells)
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size : 122 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
ab53007 stained HeLa cells at 1/100-1/250 dilution.
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