![Western blot - ATP citrate lyase antibody [EP737Y] (ab53007) image](#ab53007_WB_1.jpg)
![Immunocytochemistry/ Immunofluorescence - ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) image](#ab53007_IHC_1.jpg)
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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-ATP citrate lyase antibody [EP737Y]
See all ATP citrate lyase products (6) ...
Description
Rabbit monoclonal [EP737Y] to ATP citrate lyase
Specificity
ab53007 only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase.
Positive control
3T3 cytoplasmic cell lysate HeLa cells
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP737Y
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 100 kDa (predicted molecular weight: 122 kDa).
ICC/IF: 1/100 - 1/250.
Application notes
Is unsuitable for ,Flow Cyt or IHC-P.
Target
Function
ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Sequence similarities
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP53396
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACL antibody
- Acly antibody
- ACLY_HUMAN antibody
see all
Database links
- Entrez Gene: 47 Human
- Entrez Gene: 104112 Mouse
- Entrez Gene: 24159 Rat
- Omim: 108728 Human
- SwissProt: P53396 Human
- SwissProt: Q91V92 Mouse
- SwissProt: P16638 Rat
- Unigene: 387567 Human
see all
Anti-ATP citrate lyase antibody [EP737Y] images:
Western blot - ATP citrate lyase antibody [EP737Y] (ab53007)
![Western blot - ATP citrate lyase antibody [EP737Y] (ab53007)](/ps/datasheet/Images/53/ab53007/ab53007_WB_1.jpg)
All lanes : Anti-ATP citrate lyase antibody [EP737Y] (ab53007) at 1/1000 dilution
Lane 1 : (A) 3T3 cell lysate (untreated cells)
Lane 2 : (B) 3T3 cell lysate (insulin treated cells)
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size : 122 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
![Immunocytochemistry/ Immunofluorescence - ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)](/ps/datasheet/Images/53/ab53007/ab53007_IHC_1.jpg)
ab53007 stained HeLa cells at 1/100-1/250 dilution.
References for Anti-ATP citrate lyase antibody [EP737Y] (ab53007)
ab53007 has not yet been referenced specifically in any publications.
Publishing research using ab53007? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"