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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-ATP citrate lyase antibody
See all ATP citrate lyase products (6) ...
Description
Rabbit polyclonal to ATP citrate lyase
Tested applications
IHC-P, ELISA, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human ATP Citrate Lyase around the phosphorylation site of serine 454 (T-A-SP-F-S).
Positive control
Extracts from COS7 cells, treated with Calyculin (50nM, 30mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
ELISA: 1/5,000ELISA: 1/5,000
WB: 1/500 - 1/1000.Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Sequence similarities
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP53396
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACL antibody
- Acly antibody
- ACLY_HUMAN antibody
see all
Database links
- Entrez Gene: 47 Human
- Entrez Gene: 104112 Mouse
- Entrez Gene: 24159 Rat
- Omim: 108728 Human
- SwissProt: P53396 Human
- SwissProt: Q91V92 Mouse
- SwissProt: P16638 Rat
- Unigene: 387567 Human
see all
Anti-ATP citrate lyase antibody images:
Western blot - ATP citrate lyase antibody (ab61762)
All lanes : Anti-ATP citrate lyase antibody (ab61762) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated
with Calyculin (50nM, 30mins).
Lane 2 : Extracts from COS7 cells, treated
with Calyculin (50nM, 30mins) and the immunising peptide.
Predicted band size : 122 kDa
Observed band size : 122 kDa
Immunocytochemistry/ Immunofluorescence - ATP citrate lyase antibody (ab61762)
ICC/IF image of ab61762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61762, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-ATP citrate lyase antibody(ab61762)
IHC image of ab61762 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab61762, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-ATP citrate lyase antibody (ab61762)
ab61762 has not yet been referenced specifically in any publications.
Publishing research using ab61762? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"