Feedback Form

Western blot - ATP citrate lyase antibody (ab61762)
Western blot - ATP citrate lyase antibody (ab61762)
(enlarge)


Immunocytochemistry/ Immunofluorescence - ATP citrate lyase antibody (ab61762)
Immunocytochemistry/ Immunofluorescence - ATP citrate lyase antibody (ab61762)
(enlarge)


Product Name 

ATP citrate lyase antibody

 

See all ATP citrate lyase antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ATP citrate lyase

Immunogen 

Synthetic non-phosphopeptide derived from human ATP Citrate Lyase around the phosphorylation site of serine 454 (T-A-SP-F-S).

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ELISA, ICC/IF, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• WB

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ELISA: 1/5,000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from COS7 cells, treated with Calyculin (50nM, 30mins).

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL.

 

Alternative names ATP citrate lyase antibody (ab61762)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

47    (Human)
104112    (Mouse)
24159    (Rat)

Omim

    

108728    (Human)

SwissProt

    

P53396    (Human)
Q91V92    (Mouse)
P16638    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATP citrate lyase antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ATP citrate lyase antibody images, references, products related to ab61762 and other tools.

 

ATP citrate lyase antibody images:

Product image  Western blot - ATP citrate lyase antibody (ab61762)

Western blot - ATP citrate lyase antibody (ab61762)

All lanes : ATP citrate lyase antibody (ab61762) at 1/500 dilution

Lane 1 : Extracts from COS7 cells, treated with Calyculin (50nM, 30mins).
Lane 2 : Extracts from COS7 cells, treated with Calyculin (50nM, 30mins) and the immunising peptide.


Predicted band size : 122 kDa
Observed band size : 122 kDa

 

Product image  Immunocytochemistry/ Immunofluorescence - ATP citrate lyase antibody (ab61762)

Immunocytochemistry/ Immunofluorescence - ATP citrate lyase antibody (ab61762)

ICC/IF image of ab61762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61762, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 
 

Search PubMed (MEDLINE) for references to ATP citrate lyase

 

ATP citrate lyase antibody - more information

Customer reviews (feedback) regarding ATP citrate lyase antibody

Customer FAQs regarding ATP citrate lyase antibody

Protocols for ATP citrate lyase antibody

Price and availability of products related to ATP citrate lyase antibody

 

ATP citrate lyase antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for ATP citrate lyase antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other ATP citrate lyase Antibodies

See all ATP citrate lyase antibodies (5)...

 

ATP citrate lyase antibody - other tools:

Contact Abcam with a Technical Enquiry about ATP citrate lyase antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.