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Western blot - ATP citrate lyase antibody (ab61762)
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Immunocytochemistry/ Immunofluorescence - ATP citrate lyase antibody (ab61762)
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Product Name
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ATP citrate lyase antibody
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See all ATP citrate lyase antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATP citrate lyase
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Immunogen
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Synthetic non-phosphopeptide derived from human ATP Citrate Lyase around the phosphorylation site of serine 454 (T-A-SP-F-S).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5,000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from COS7 cells, treated with Calyculin (50nM, 30mins).
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATP citrate lyase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATP citrate lyase antibody images, references, products related to ab61762 and other tools.
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ATP citrate lyase antibody images:
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 Western blot - ATP citrate lyase antibody (ab61762)
All lanes : ATP citrate lyase antibody (ab61762) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated
with Calyculin (50nM, 30mins).
Lane 2 : Extracts from COS7 cells, treated
with Calyculin (50nM, 30mins) and the immunising peptide.
Predicted band size : 122 kDa
Observed band size : 122 kDa
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Immunocytochemistry/ Immunofluorescence - ATP citrate lyase antibody (ab61762)
ICC/IF image of ab61762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61762, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to ATP citrate lyase
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ATP citrate lyase antibody - more information
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Customer reviews (feedback) regarding ATP citrate lyase antibody |
Customer FAQs regarding ATP citrate lyase antibody |
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