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Immunohistochemistry (Paraffin-embedded sections) - ATP citrate lyase (phospho S454) antibody (ab59297)
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Product Name
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ATP citrate lyase (phospho S454) antibody
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See all ATP citrate lyase antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATP citrate lyase (phospho S454)
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Immunogen
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Synthetic phosphopeptide derived from human ATP Citrate Lyase around the phosphorylation site of serine 454 (T-A-SP-F-S).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects endogenous levels of ATP Citrate Lyase only when phosphorylated at serine 454.
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Tested applications
(see key)
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ELISA, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human colon carcinoma tissue
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL.
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ATP citrate lyase (phospho S454) antibody (ab59297) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATP citrate lyase (phospho S454) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATP citrate lyase (phospho S454) antibody images, references, products related to ab59297 and other tools.
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ATP citrate lyase (phospho S454) antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - ATP citrate lyase (phospho S454) antibody (ab59297)
Ab59297 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.
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Search PubMed (MEDLINE) for references to ATP citrate lyase
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ATP citrate lyase (phospho S454) antibody - more information
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Customer reviews (feedback) regarding ATP citrate lyase (phospho S454) antibody |
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ATP citrate lyase (phospho S454) antibody - related products:
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ATP citrate lyase (phospho S454) antibody - other tools:
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