Overview

  • Product nameAnti-ATP5F1 antibody
    See all ATP5F1 primary antibodies
  • Description
    Rabbit polyclonal to ATP5F1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST) of human ATP5F1 (NP_001679).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similaritiesBelongs to the eukaryotic ATPase B chain family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT5F1_HUMAN antibody
    • ATP synthase B chain mitochondrial antibody
    • ATP synthase subunit b antibody
    • ATP synthase subunit b mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody
    • ATP5F1 antibody
    • ATPase subunit b antibody
    • Cell proliferation inducing protein 47 antibody
    • H+ ATP synthase subunit b antibody
    • MGC24431 antibody
    • mitochondrial antibody
    • PIG47 antibody
    see all

Anti-ATP5F1 antibody images

  • Anti-ATP5F1 antibody (ab84625) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATP5F1 antibody (ab84625)

ab84625 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (7% GEL)
Sample Human Cell lysate - other (H23)
Specification H23
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Oct 31 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"