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Overview

  • Product nameAnti-ATP5F1 antibodySee all ATP5F1 primary antibodies ...
  • Description
    Rabbit polyclonal to ATP5F1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST) of human ATP5F1 (NP_001679).

  • Positive control721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similaritiesBelongs to the eukaryotic ATPase B chain family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane.

    • Target information above from: UniProt accession P24539 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      AT5F1_HUMAN antibodyATP synthase B chain mitochondrial antibodyATP synthase subunit b antibody
      ATP synthase subunit b mitochondrial antibodyATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, antibodyATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibodyATP5F1 antibodyATPase subunit b antibodyCell proliferation inducing protein 47 antibodyH+ ATP synthase subunit b antibodyMGC24431 antibodymitochondrial antibodyPIG47 antibody
    see all

Anti-ATP5F1 antibody images

  • Western blot - ATP5F1 antibody (ab84625)
    Western blot - ATP5F1 antibody (ab84625)
    Anti-ATP5F1 antibody (ab84625) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATP5F1 antibody (ab84625)

ab84625 has not yet been referenced specifically in any publications.

Publishing research using ab84625 ? Please let us know so that we can cite the reference in this datasheet

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