Overview

  • Product nameAnti-ATP5G2 antibodySee all ATP5G2 primary antibodies ...
  • Description
    Rabbit polyclonal to ATP5G2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide: LLSRPLSAVV LKRPEILTDE SLSSLAVSCP LTSLVSSRSF QTSAISRDID , corresponding to N terminal amino acids 36 - 85 of Human ATP5G2 (NP_001002031)

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-ATP5G2 antibody images

  • Anti-ATP5G2 antibody (ab80325) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATP5G2 antibody (ab80325)

This product has been referenced in:
  • Chen K  et al. AMP activated protein kinase is indispensable for myocardial adaptation to caloric restriction in mice. PLoS One 8:e59682 (2013). WB ; Mouse . Read more (PubMed: 23527250) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 20 µg
Specification 293T
Treatment HEK 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 01 2011

Application Western blot
Sample Mouse Cell lysate - other (Liver Mitochondria)
Loading amount 20 µg
Specification Liver Mitochondria
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"