Overview
- Product nameAnti-ATP5G2 antibodySee all ATP5G2 primary antibodies ...
- DescriptionRabbit polyclonal to ATP5G2
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Zebrafish, Orangutan - Immunogen
Synthetic peptide: LLSRPLSAVV LKRPEILTDE SLSSLAVSCP LTSLVSSRSF QTSAISRDID , corresponding to N terminal amino acids 36 - 85 of Human ATP5G2 (NP_001002031)
- Positive controlFetal heart lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab80325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
- Sequence similaritiesBelongs to the ATPase C chain family.
- Cellular localizationMitochondrion membrane.
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Database links
- Entrez Gene: 516 Human
- Entrez Gene: 518 Human
- Entrez Gene: 517 Human
- Entrez Gene: 517 Human
- Entrez Gene: 67942 Mouse
- Entrez Gene: 228033 Mouse
- Entrez Gene: 11951 Mouse
- Entrez Gene: 100172599 Orangutan
- Entrez Gene: 29754 Rat
- Entrez Gene: 171082 Rat
- Entrez Gene: 114630 Rat
- Omim: 603193 Human
- SwissProt: P48201 Human
- SwissProt: Q06055 Human
- SwissProt: P05496 Human
- SwissProt: Q06055 Human
- SwissProt: Q9CR84 Mouse
- SwissProt: P56384 Mouse
- SwissProt: P56383 Mouse
- SwissProt: Q5RAP9 Orangutan
- SwissProt: Q71S46 Rat
- SwissProt: Q06645 Rat
- SwissProt: Q06646 Rat
- Unigene: 80986 Human
- Unigene: 429 Human
- Unigene: 524464 Human
- Unigene: 10314 Mouse
- Unigene: 381662 Mouse
- Unigene: 2966 Mouse
- Unigene: 258 Mouse
- Unigene: 154476 Rat
- Unigene: 3357 Rat
- Unigene: 127811 Rat
- Unigene: 29258 Rat
- Unigene: 2180 Rat
see all
Target information above from: UniProt accession
Q06055
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AT5G2_HUMAN antibodyATP synthase H+ transporting mitochondrial F0 complex subunit c subunit 9 isoform 2 antibodyATP synthase H+ transporting mitochondrial F0 complex subunit C2 antibody
- ATP synthase H+ transporting mitochondrial F0 complex subunit C2 subunit 9 antibodyATP synthase lipid binding protein mitochondrial antibodyATP synthase lipid-binding protein antibodyATP synthase proteolipid P2 antibodyATP5G2 antibodyATPase protein 9 antibodyATPase subunit c antibodymitochondrial antibodyMitochondrial ATP synthase subunit C subunit 9 isoform 2 antibodyOTTHUMP00000164642 antibody
see all
Anti-ATP5G2 antibody images
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Western blot - ATP5G2 antibody (ab80325)Anti-ATP5G2 antibody (ab80325) at 1 µg/ml + fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 15 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ATP5G2 antibody (ab80325)
ab80325 has not yet been referenced specifically in any publications.
