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Anti-ATP5H antibody [7F9BG1 ] (ab110275)

MSCatalog No. MS504

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Overview

Product name

Anti-ATP5H antibody [7F9BG1 ]
See all ATP5H products (3) ...

Description

Mouse monoclonal [7F9BG1 ] to ATP5H

Tested applications

WB, ICC/IF, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

Bovine Complex V

Positive control

Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Near homogeneity as judged by SDS-PAGE. ab110275 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

7F9BG1

Isotype

IgG2b

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex V
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

  • Western blot - ATP5H antibody [7F9BG1 ] (ab110275)Western blot - ATP5H antibody [7F9BG1 ] (ab110275) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - ATP5H antibody [7F9BG1 ] (ab110275)Immunocytochemistry/ Immunofluorescence - ATP5H antibody [7F9BG1 ] (ab110275) image (enlarge)

  • Flow Cytometry - ATP5H antibody [7F9BG1 ] (ab110275)Flow Cytometry - ATP5H antibody [7F9BG1 ] (ab110275) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Sequence similarities

Belongs to the ATPase d subunit family.

Cellular localization

Mitochondrion. Mitochondrion inner membrane.

Target information above from: UniProt accessionO75947 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • ATP synthase subunit d antibody
  • ATP synthase subunit d, mitochondrial antibody
  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
  • ATP5H antibody
  • ATP5H_HUMAN antibody
  • ATP5JD antibody
  • ATPase subunit d antibody
  • ATPQ antibody
  • MS504 antibody
see all

Database links

see all

Anti-ATP5H antibody [7F9BG1 ] images:

  Western blot - ATP5H antibody [7F9BG1 ] (ab110275)

Western blot - ATP5H antibody [7F9BG1 ] (ab110275)

All lanes : Anti-ATP5H antibody [7F9BG1 ] (ab110275) at 1/1 dilution

Lane 1 : Human heart mitochondria at 10 µg
Lane 2 : Bovine heart mitochondria at 4 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg


Predicted band size : 18 kDa

  Immunocytochemistry/ Immunofluorescence - ATP5H antibody [7F9BG1 ] (ab110275)

Immunocytochemistry/ Immunofluorescence - ATP5H antibody [7F9BG1 ] (ab110275)

Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).

  Flow Cytometry - ATP5H antibody [7F9BG1 ] (ab110275)

Flow Cytometry - ATP5H antibody [7F9BG1 ] (ab110275)

HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

References for Anti-ATP5H antibody [7F9BG1 ] (ab110275)

This product has been referenced in:

  • Mayr JA  et al. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit. Hum Mol Genet 19:3430-9 (2010).Read more (PubMed: 20566710) »
  • Sansanwal P  et al. Mitochondrial autophagy promotes cellular injury in nephropathic cystinosis. J Am Soc Nephrol 21:272-83 (2010).Read more (PubMed: 19959713) »

See all 7 publications for this product

Publishing research using ab110275? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"