Anti-ATP5J antibody [10C6AC9 ] (ab110279)
- Product nameAnti-ATP5J antibody [10C6AC9 ]See all ATP5J primary antibodies ...
- DescriptionMouse monoclonal [10C6AC9 ] to ATP5J
- Tested applicationsWB, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Human
Recombinant Human ATP5J protein (UniProt ID: P18859).
- Positive controlWB: Isolated mitochondria from Human heart lysate. ICc/IF: Cultured Human embryonic lung-derived fibroblasts (strain MRC5) Flow Cyt: HL-60 cells
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPreservative: 0.02% Sodium azide
- Concentration information loading...
- Purityby SDS-PAGE
- Purification notesThe purity of ab110279 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
- Clonality Monoclonal
- Clone number10C6AC9
- Light chain typekappa
Our Abpromise guarantee covers the use of ab110279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa.|
|Flow Cyt||Flow Cyt: Use a concentration of 1 µg/ml.|
|ICC/IF||ICC/IF: Use a concentration of 1 µg/ml.|
- FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
- Sequence similaritiesBelongs to the eukaryotic ATPase subunit F6 family.
- Cellular localizationMitochondrion. Mitochondrion inner membrane.
- ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibodyATP synthase-coupling factor 6 antibodyATP synthase-coupling factor 6, mitochondrial antibody
- ATP5 antibodyATP5A antibodyATP5J antibodyATP5J_HUMAN antibodyATPase subunit F6 antibodyATPM antibodyCF6 antibodyF6 antibodymitochondrial antibody
Anti-ATP5J antibody [10C6AC9 ] images
Anti-ATP5J antibody [10C6AC9 ] (ab110279) at 1 µg/ml + Isolated mitochondria from Human heart lysate at 5 µg
Predicted band size : 13 kDa
Mitochondrial localization of ATP synthase visualized by immunocytochemistry using ab110279.
Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110279 (1 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
HL-60 cells were stained with 1 µg/mL ab110279 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
All lanes : Anti-ATP5J antibody [10C6AC9 ] (ab110279)
Lane 1 : human heart homogenate at 20 µg
Lane 2 : HepG2 cell lysate at 20 µg
Lane 3 : human liver mitochondrial extraction at 10 µg
Lane 4 : bovine heart mitochondrial extraction at 10 µg
Lane 5 : human heart mitochondrial extraction at 10 µg
Lane 6 : human liver homogenate at 20 µg
Predicted band size : 13 kDa
References for Anti-ATP5J antibody [10C6AC9 ] (ab110279)
This product has been referenced in:
- Mayr JA et al. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit. Hum Mol Genet 19:3430-9 (2010). Read more (PubMed: 20566710) »