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MitoSciences (MS508)

Anti-ATP5J antibody [10C6AC9 ] (ab110279)

Submit an Abreview Q&A (5)Specific References (1)

Overview

  • Product nameAnti-ATP5J antibody [10C6AC9 ]See all ATP5J primary antibodies ...
  • Description
    Mouse monoclonal [10C6AC9 ] to ATP5J
  • Tested applicationsWB, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human ATP5J protein (UniProt ID: P18859).

  • Positive controlWB: Isolated mitochondria from Human heart lysate. ICc/IF: Cultured Human embryonic lung-derived fibroblasts (strain MRC5) Flow Cyt: HL-60 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab110279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa.
Flow Cyt Flow Cyt: Use a concentration of 1 µg/ml.
ICC/IF ICC/IF: Use a concentration of 1 µg/ml.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
  • Sequence similaritiesBelongs to the eukaryotic ATPase subunit F6 family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane.

    • Target information above from: UniProt accession P18859 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibodyATP synthase-coupling factor 6 antibodyATP synthase-coupling factor 6, mitochondrial antibody
        ATP5 antibodyATP5A antibodyATP5J antibodyATP5J_HUMAN antibodyATPase subunit F6 antibodyATPM antibodyCF6 antibodyF6 antibodymitochondrial antibody
      see all

    Anti-ATP5J antibody [10C6AC9 ] images

    • Western blot - Anti-ATP5J antibody (ab110279)
      Western blot - Anti-ATP5J antibody (ab110279)
      Anti-ATP5J antibody [10C6AC9 ] (ab110279) at 1 µg/ml + Isolated mitochondria from Human heart lysate at 5 µg

      Predicted band size : 13 kDa
    • Immunocytochemistry/ Immunofluorescence - ATP5J antibody (ab110279)
      Immunocytochemistry/ Immunofluorescence - ATP5J antibody (ab110279)
      Mitochondrial localization of ATP synthase visualized by immunocytochemistry using ab110279.
      Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110279 (1 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
    • Flow Cytometry - ATP5J antibody (ab110279)
      Flow Cytometry - ATP5J antibody (ab110279)
      HL-60 cells were stained with 1 µg/mL ab110279 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
    • Western blot - Anti-ATP5J antibody (ab110279)
      Western blot - Anti-ATP5J antibody (ab110279)
      All lanes : Anti-ATP5J antibody [10C6AC9 ] (ab110279)

      Lane 1 : human heart homogenate at 20 µg
      Lane 2 : HepG2 cell lysate at 20 µg
      Lane 3 : human liver mitochondrial extraction at 10 µg
      Lane 4 : bovine heart mitochondrial extraction at 10 µg
      Lane 5 : human heart mitochondrial extraction at 10 µg
      Lane 6 : human liver homogenate at 20 µg


      Predicted band size : 13 kDa

    References for Anti-ATP5J antibody [10C6AC9 ] (ab110279)

    This product has been referenced in:
    • Mayr JA  et al. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit. Hum Mol Genet 19:3430-9 (2010). Read more (PubMed: 20566710) »

    See 1 Publication for this product

    Publishing research using ab110279 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab110279 Submit a Question for ab110279

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

    10
    Votes: Highest First

    Dear All,

    we have a customer who is looking for an anti mitochondria marker specific for human and that doesn't react with mouse.

    The customer has to identify human cells injected into mouse.

    I checked in your website but I did...

    Read More

    Thank you for your email, I am sorry for the delay in getting back to you.

    I have a couple of antibodies your customer may be interested in:

    Anti-Frataxin antibody [18A5DB1] (ab110328)

    and

    Anti-ATP5J antibody [10C6AC9] ...

    Read More

    Je vous envoie en fichier joint une image d'un résultat de western blot et immunodétection avec l'anticorps anti-Atp6 (F6, ab110279/MS508). Ce résultat est totalement reproductible, que ce soit sur des extraits de protéines totales, mitochondriales, dans ...

    Read More

    Merci beaucoup pour votre patience. Le laboratoire avait retesté cet anticorps sur différents tissus. Veuillez trouver les résultats dans le pièce jointe. Effectivement nous sommes très confiantes que cet anticorps reconnaît le ATP5J, car il était dévelop...

    Read More

    Used the antibody ab110279/MS508 for many Western blots (about 10 times) and always a band at about 25kD shows. Samples are of human cancer cells, reduced and denatured

    Je suis désolée d'apprendre que ce tube de l'ab110279 ne donne pas de bons résultats en Western blot.  Veuillez trouver ci-joint le questionnaire discuté au téléphone. Veuillez le nous retourner avec une image du résultat. Comme discuté aussi, je vais ens...

    Read More

    Thank you for your reply. We will try to use this ATP5J antibody (MS508) for mouse lysates. Would you give us the discount code for the next purchase, please? After getting that, we will order MS508 antibody. Thank you,  

    DISCOUNT CODE: XXXXXXXXX Expiration date: 15-01-2012 I am very pleased to hear you would like to accept our offer and test ab110279 in Mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pleas...

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    I am interested in your antibody ATP synthase subunit F6 monoclonal antibody (MS508). But my sample is mouse liver and mouse kidney. Would we use this antibody for western blot of my mouse samples? Thank you,  

    Thank you for contacting us. We have not tested this antibody with mouse lysates yet so we unfortunately are unable to share any tested data. The mouse and human protein have 80% homology so it is more likely that this antibody could detect the mo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"