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Product name
Anti-ATP5J antibody
See all ATP5J products (2) ...
Description
Mouse monoclonal to ATP5J
Tested applications
WB, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant Human ATP5J protein (UniProt ID: P18859).
Positive control
WB: Isolated mitochondria from Human heart lysate. ICc/IF: Cultured Human embryonic lung-derived fibroblasts (strain MRC5) Flow Cyt: HL-60 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
by SDS-PAGE
Purification notes
The purity of ab110279 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex V
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa.
Flow Cyt: Use a concentration of 1 µg/ml.
ICC/IF: Use a concentration of 1 µg/ml.
Target
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
Sequence similarities
Belongs to the eukaryotic ATPase subunit F6 family.
Cellular localization
Mitochondrion. Mitochondrion inner membrane.
Target information above from: UniProt accessionP18859
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CF6 antibody
- mitochondrial antibody
- ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
see all
Database links
- Entrez Gene: 522 Human
- SwissProt: P18859 Human
- Unigene: 246310 Human
Anti-ATP5J antibody images:
Western blot - Anti-ATP5J antibody (ab110279)
Anti-ATP5J antibody (ab110279) at 1 µg/ml + Isolated mitochondria from Human heart lysate at 5 µg
Predicted band size : 13 kDa
Immunocytochemistry/ Immunofluorescence - ATP5J antibody (ab110279)
Mitochondrial localization of ATP synthase visualized by immunocytochemistry using ab110279.
Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110279 (1 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
Flow Cytometry - ATP5J antibody (ab110279)
HL-60 cells were stained with 1 µg/mL ab110279 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
Western blot - Anti-ATP5J antibody (ab110279)
All lanes : Anti-ATP5J antibody (ab110279)
Lane 1 : human heart homogenate at 20 µg
Lane 2 : HepG2 cell lysate at 20 µg
Lane 3 : human liver mitochondrial extraction at 10 µg
Lane 4 : bovine heart mitochondrial extraction at 10 µg
Lane 5 : human heart mitochondrial extraction at 10 µg
Lane 6 : human liver homogenate at 20 µg
Predicted band size : 13 kDa
Although the ATP5J gene has alternatively spliced transcript variants which encodes different isoforms, the MW of the isoforms should within 10~12kDa. The high MW band detected in liver tissues as well as in bovine heart may be a structural complex which involves ATP5J.
References for Anti-ATP5J antibody (ab110279)
This product has been referenced in:
- Mayr JA et al. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit. Hum Mol Genet 19:3430-9 (2010).Read more (PubMed: 20566710) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"